Department of Plasma Bioscience and Display, Kwangwoon University, Seoul, Republic of Korea.
Cell Death Dis. 2013 May 23;4(5):e642. doi: 10.1038/cddis.2013.168.
The distinctive cellular and mitochondrial dysfunctions of two human lung cancer cell lines (H460 and HCC1588) from two human lung normal cell lines (MRC5 and L132) have been studied by dielectric barrier discharge (DBD) plasma treatment. This cytotoxicity is exposure time-dependent, which is strongly mediated by the large amount of H2O2 and NOx in culture media generated by DBD nonthermal plasma. It is found that the cell number of lung cancer cells has been reduced more than that of the lung normal cells. The mitochondrial vulnerability to reactive species in H460 may induce distinctively selective responses. Differential mitochondrial membrane potential decrease, mitochondrial enzymatic dysfunction, and mitochondrial morphological alteration are exhibited in two cell lines. These results suggest the nonthermal plasma treatment as an efficacious modality in lung cancer therapy.
已通过介质阻挡放电（DBD）等离子体处理研究了两种人肺癌细胞系（H460 和 HCC1588）与两种人正常肺细胞系（MRC5 和 L132）之间独特的细胞和线粒体功能障碍。这种细胞毒性与暴露时间有关，这主要是由 DBD 非热等离子体在培养基中产生的大量 H2O2 和 NOx 介导的。结果发现，肺癌细胞的数量减少超过了正常肺细胞的数量。H460 中活性物质对线粒体的脆弱性可能会引起明显的选择性反应。两种细胞系均表现出不同的线粒体膜电位下降、线粒体酶功能障碍和线粒体形态改变。这些结果表明，非热等离子体处理是一种有效的肺癌治疗方法。
