Choi Hyeongwon, Choi Eun Ha, Kim Kyung Sook
Department of Genetic Engineering, College of Life Science and Graduate School of Biotechnology, Kyung Hee University, Yongin, Korea.
Lutronic R&D Center, 219, Sowon-ro, Deogyang-gu, Goyang-si, Gyeonggi-do, Korea.
Microsc Res Tech. 2017 Oct;80(10):1078-1084. doi: 10.1002/jemt.22902. Epub 2017 Jun 22.
Mechanical properties of a single cell are closely related to the fate and functions of the cell. Changes in mechanical properties may cause diseases or cell apoptosis. Selective cytotoxic effects of nonthermal atmospheric pressure micro-dielectric barrier discharge (DBD) plasma have been demonstrated on cancer cells. In this work, changes in the mechanical properties of a single cell induced by nonthermal atmospheric pressure micro-DBD plasma were investigated using atomic force microscopy (AFM). Two cervical cancer cell lines (HeLa and SiHa) and normal human fibroblast cells (HFBs) were exposed to micro-DBD plasma for various exposure times. The elasticity of a single cell was determined by force-distance curve measurement using AFM. Young's modulus was decreased by plasma treatment for all cells. The Young's modulus of plasma-treated HeLa cells was decreased by 75% compared to nontreated HeLa cells. In SiHa cells and HFBs, elasticity was decreased slightly. Chemical changes induced by the plasma treatment, which were observed by Raman spectroscopy, were also significant in HeLa cells compared to SiHa cells and HFBs. These results suggested that the molecular changes induced by micro-DBD plasma were related to cell mechanical changes.
单个细胞的力学性质与细胞的命运和功能密切相关。力学性质的变化可能导致疾病或细胞凋亡。非热大气压微介电阻挡放电（DBD）等离子体对癌细胞具有选择性细胞毒性作用。在本研究中，使用原子力显微镜（AFM）研究了非热大气压微DBD等离子体诱导的单个细胞力学性质的变化。将两种宫颈癌细胞系（HeLa和SiHa）和正常人成纤维细胞（HFBs）暴露于微DBD等离子体不同时间。通过使用AFM测量力-距离曲线来确定单个细胞的弹性。所有细胞经等离子体处理后杨氏模量均降低。与未处理的HeLa细胞相比，经等离子体处理的HeLa细胞的杨氏模量降低了75%。在SiHa细胞和HFBs中，弹性略有降低。通过拉曼光谱观察到的等离子体处理引起的化学变化，在HeLa细胞中也比在SiHa细胞和HFBs中更为显著。这些结果表明，微DBD等离子体诱导的分子变化与细胞力学变化有关。
