人脐带血血清：用于培养人多能间充质基质细胞的胎牛血清有效替代品
Human Umbilical Cord Blood Serum: Effective Substitute of Fetal Bovine Serum for Culturing of Human Multipotent Mesenchymal Stromal Cells.
作者信息
Romanov Yu A, Balashova E E, Volgina N E, Kabaeva N V, Dugina T N, Sukhikh G T
机构信息
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation, Moscow, Russia.
CryoCenter Cord Blood Bank, Moscow, Russia.
出版信息
Bull Exp Biol Med. 2017 Feb;162(4):528-533. doi: 10.1007/s10517-017-3654-9. Epub 2017 Feb 27.
Optimal conditions for culturing of multipotent mesenchymal stromal cells in the presence of pooled umbilical cord blood serum were determined. It was found that umbilical cord blood serum in a concentration range of 1-10% effectively supported high viability and proliferative activity of cells with unaltered phenotype and preserved multilineage differentiation capacity. The proposed approach allows avoiding the use of xenogenic animal sera for culturing of multipotent mesenchymal stromal cells and creates prerequisites for designing and manufacturing safe cellular and/or acellular products for medical purposes.
确定了在混合脐带血血清存在下培养多能间充质基质细胞的最佳条件。发现浓度范围为1-10%的脐带血血清能有效支持细胞的高活力和增殖活性，细胞表型未改变且保留多系分化能力。所提出的方法避免了使用异种动物血清来培养多能间充质基质细胞，并为设计和制造用于医疗目的的安全细胞和/或无细胞产品创造了前提条件。
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