Muturi Ephantus J, Ramirez Jose L, Rooney Alejandro P, Kim Chang-Hyun
Crop Bioprotection Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Peoria, IL United States of America.
Illinois Natural History Survey, University of Illinois at Urbana-Champaign, Champaign IL. United States of America.
PLoS Negl Trop Dis. 2017 Feb 28;11(2):e0005377. doi: 10.1371/journal.pntd.0005377. eCollection 2017 Feb.
The composition and structure of microbial communities that inhabit the mosquito midguts are poorly understood despite their well-documented potential to impede pathogen transmission.
METHODOLOGY/PRINCIPAL FINDINGS: We used MiSeq sequencing of the 16S rRNA gene to characterize the bacterial communities of field-collected populations of 12 mosquito species. After quality filtering and rarefaction, the remaining sequences were assigned to 181 operational taxonomic units (OTUs). Approximately 58% of these OTUs occurred in at least two mosquito species but only three OTUs: Gluconobacter (OTU 1), Propionibacterium (OTU 9), and Staphylococcus (OTU 31) occurred in all 12 mosquito species. Individuals of different mosquito species shared similar gut microbiota and it was common for individuals of the same species from the same study site and collection date to harbor different gut microbiota. On average, the microbiota of Aedes albopictus was the least diverse and significantly less even compared to Anopheles crucians, An. quadrimaculatus, Ae. triseriatus, Ae. vexans, Ae. japonicus, Culex restuans, and Culiseta inornata. The microbial community of Cx. pipiens and Ae. albopictus differed significantly from all other mosquitoes species and was primarily driven by the dominance of Wolbachia.
These findings expand the range of mosquito species whose gut microbiota has been characterized and sets the foundation for further studies to determine the influence of these microbiota on vector susceptibility to pathogens.
尽管有充分证据表明栖息在蚊子中肠的微生物群落有阻碍病原体传播的潜力，但对其组成和结构仍知之甚少。
方法/主要发现：我们使用16S rRNA基因的MiSeq测序来表征12种野外采集的蚊子种群的细菌群落。经过质量过滤和稀疏化处理后，剩余序列被归为181个操作分类单元（OTU）。这些OTU中约58%出现在至少两种蚊子物种中，但只有三个OTU：葡糖杆菌（OTU 1）、丙酸杆菌（OTU 9）和葡萄球菌（OTU 31）出现在所有12种蚊子物种中。不同蚊子物种的个体共享相似的肠道微生物群，并且来自同一研究地点和采集日期的同一物种个体携带不同的肠道微生物群是很常见的。平均而言，白纹伊蚊的微生物群多样性最低，与十字疟蚊、四斑按蚊、三带喙库蚊、骚扰阿蚊、日本伊蚊、致倦库蚊和无饰库蚊相比，均匀度也显著更低。尖音库蚊和白纹伊蚊的微生物群落与所有其他蚊子物种有显著差异，主要由沃尔巴克氏体的优势地位驱动。
这些发现扩展了已表征肠道微生物群的蚊子物种范围，并为进一步研究确定这些微生物群对病媒对病原体易感性的影响奠定了基础。
