Che Long, Yang ZhenGuo, Xu MengMeng, Xu ShengYu, Che LianQiang, Lin Yan, Fang ZhengFeng, Feng Bin, Li Jian, Chen DaiWen, Wu De
Key Laboratory of Animal Disease-Resistance Nutrition and Feed Science, Ministry of Agriculture, P. R. China, Institute of Animal Nutrition, Sichuan Agricultural University, Chengdu, 611130, China.
BMC Genomics. 2017 Feb 28;18(1):213. doi: 10.1186/s12864-017-3601-1.
Intra-uterine growth restriction (IUGR) and fetal overgrowth increase risks to postnatal health. Maternal nutrition is the major intrauterine environmental factor that alters fetal weight. However, the mechanisms underlying the effects of maternal nutrition on fetal development are not entirely clear. We developed a pig model, and using isobaric tags for relative and absolute quantification (iTRAQ), we investigated alterations in the placental proteome of gilts on a normal-energy-intake (Con) and high-energy-intake (HE) diet.
In the Con group, heavy and light fetuses were found at the tubal and cervical ends of the uterus respectively at 90 d of gestation. Moreover, the heavy fetuses had a higher glucose concentration than the light fetuses. However, a higher uniformity was noted in the HE group. Placental promoters between these two positions indicated that 78 and 50 differentially expressed proteins were detected in the Con and HE groups respectively. In the Con group, these proteins were involved in lipid metabolism (HADHA, AACS, CAD), nutrient transport (GLUT, SLC27A1), and energy metabolism (NDUFV1, NDUFV2, ATP5C1). However, in the HE group they mainly participated in transcriptional and translational regulation, and intracellular vesicular transport.
Our findings revealed that maternal nutrition may alter birth weight mainly through the modulation of placental lipid and energy metabolism, which also provides a possible mechanism to explain the higher uniformity of fetal weight in gilts fed a HE diet.
宫内生长受限（IUGR）和胎儿过度生长会增加出生后健康风险。母体营养是改变胎儿体重的主要宫内环境因素。然而，母体营养对胎儿发育影响的潜在机制尚不完全清楚。我们建立了一个猪模型，并使用相对和绝对定量的等压标签（iTRAQ），研究了正常能量摄入（Con）和高能量摄入（HE）日粮的后备母猪胎盘蛋白质组的变化。
在Con组中，妊娠90天时，在子宫的输卵管端和宫颈端分别发现了较重和较轻的胎儿。此外，较重胎儿的葡萄糖浓度高于较轻胎儿。然而，HE组的胎儿体重均匀性更高。这两个位置之间的胎盘启动子表明，在Con组和HE组中分别检测到78种和50种差异表达蛋白。在Con组中，这些蛋白质参与脂质代谢（HADHA、AACS、CAD）、营养物质转运（GLUT、SLC27A1）和能量代谢（NDUFV1、NDUFV2、ATP5C1）。然而，在HE组中，它们主要参与转录和翻译调控以及细胞内囊泡运输。
我们的研究结果表明，母体营养可能主要通过调节胎盘脂质和能量代谢来改变出生体重，这也为解释饲喂HE日粮的后备母猪胎儿体重更高的均匀性提供了一种可能的机制。
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