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消除消费者担忧和监管规则：CRISPR/Cas核糖核蛋白复合物在谷物基因组编辑中的高效应用

Knocking out consumer concerns and regulator's rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals.

作者信息

Wolter Felix, Puchta Holger

机构信息

Botanical Institute, Karlsruhe Institute of Technology, Fritz-Haber-Weg 4, Karlsruhe, 76131, Germany.

出版信息

Genome Biol. 2017 Feb 28;18(1):43. doi: 10.1186/s13059-017-1179-1.

DOI:10.1186/s13059-017-1179-1
PMID:28245842
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5330017/
Abstract

Selection-free genome editing using Cas9 ribonucleoprotein embryo bombardment has been achieved for maize and wheat. This is a breakthrough that should make new breeding technologies more acceptable for worldwide use.

摘要

利用Cas9核糖核蛋白胚胎轰击技术实现了玉米和小麦的无选择基因组编辑。这是一项突破，有望使新的育种技术在全球范围内更易于被接受。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09f1/5330017/310ce9629e09/13059_2017_1179_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09f1/5330017/310ce9629e09/13059_2017_1179_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/09f1/5330017/310ce9629e09/13059_2017_1179_Fig1_HTML.jpg

相似文献

[1]
Knocking out consumer concerns and regulator's rules: efficient use of CRISPR/Cas ribonucleoprotein complexes for genome editing in cereals.

Genome Biol. 2017-2-28

[2]
Genome Editing by CRISPR/Cas9 in Sorghum Through Biolistic Bombardment.

Methods Mol Biol. 2019

[3]
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes.

Nat Commun. 2017-1-18

[4]
Genome editing in cereal crops: an overview.

Transgenic Res. 2021-8

[5]
CRISPR/Cas9: An RNA-guided highly precise synthetic tool for plant genome editing.

J Cell Physiol. 2018-3

[6]
Genome edited wheat- current advances for the second green revolution.

Biotechnol Adv. 2022-11

[7]
Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes.

Nat Commun. 2016-11-16

[8]
[Prospects for application of breakthrough technologies in breeding: The CRISPR/Cas9 system for plant genome editing].

Genetika. 2016-7

[9]
Genome Editing in Chlamydomonas reinhardtii Using Cas9-gRNA Ribonucleoprotein Complex: A Step-by-Step Guide.

Methods Mol Biol. 2023

[10]
CRISPR/Cas9: an advanced tool for editing plant genomes.

Transgenic Res. 2016-10

引用本文的文献

[1]
Advantages of Mutant Generation by Genome Rearrangements of Non-Conventional Yeast via Direct Nuclease Transfection.

Genes Cells. 2025-3

[2]
Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses.

Plants (Basel). 2024-6-24

[3]
Leveraging the sugarcane CRISPR/Cas9 technique for genetic improvement of non-cultivated grasses.

Front Plant Sci. 2024-3-27

[4]
First Report on Mesophyll Protoplast Isolation and Regeneration System for the Species.

Plants (Basel). 2023-12-21

[5]
An efficient method for protoplast-mediated production of transformed castor bean (Ricinus communis) lines.

BMC Res Notes. 2023-7-6

[6]
Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants.

Front Plant Sci. 2023-4-18

[7]
Accelerated Breeding for (Sunflower) through Doubled Haploidy: An Insight on Past and Future Prospects in the Era of Genome Editing.

Plants (Basel). 2023-1-20

[8]
Non-GM Genome Editing Approaches in Crops.

Front Genome Ed. 2021-12-15

[9]
Gene Editing Technologies for Sugarcane Improvement: Opportunities and Limitations.

Sugar Tech. 2022

[10]
Random mutagenesis in vegetatively propagated crops: opportunities, challenges and genome editing prospects.

Mol Biol Rep. 2022-6

本文引用的文献

[1]
Efficient DNA-free genome editing of bread wheat using CRISPR/Cas9 ribonucleoprotein complexes.

Nat Commun. 2017-1-18

[2]
From classical mutagenesis to nuclease-based breeding - directing natural DNA repair for a natural end-product.

Plant J. 2017-5

[3]
Genome editing in maize directed by CRISPR-Cas9 ribonucleoprotein complexes.

Nat Commun. 2016-11-16

[4]
Targeted modification of plant genomes for precision crop breeding.

Biotechnol J. 2017-1

[5]
Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA.

Nat Commun. 2016-8-25

[6]
Revolutionizing plant biology: multiple ways of genome engineering by CRISPR/Cas.

Plant Methods. 2016-1-28

[7]
DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins.

Nat Biotechnol. 2015-10-19

[8]
Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew.

Nat Biotechnol. 2014-7-20

[9]
Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins.

Genome Res. 2014-4-2

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