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通过双单倍体进行向日葵加速育种:基因组编辑时代的过去与未来前景洞察

Accelerated Breeding for (Sunflower) through Doubled Haploidy: An Insight on Past and Future Prospects in the Era of Genome Editing.

作者信息

Mabuza Londiwe M, Mchunu Nokuthula P, Crampton Bridget G, Swanevelder Dirk Z H

机构信息

Biotechnology Platform, Agricultural Research Council, Onderstepoort Campus, Onderstepoort, Pretoria 0110, South Africa.

Department of Plant Sciences, Faculty of Natural and Agricultural Sciences, University of Pretoria, Private Bag X20, Pretoria 0028, South Africa.

出版信息

Plants (Basel). 2023 Jan 20;12(3):485. doi: 10.3390/plants12030485.


DOI:10.3390/plants12030485
PMID:36771570
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9921946/
Abstract

The aim of any breeding process is to fully express the targeted, superior/desirable parent characteristic in the progeny. Hybrids are often used in this dynamic, and complex process for which homozygous parents-which may require up to eight generations of back crossing and selection-are required. Doubled haploid (DH) technologies can facilitate the production of true breeding lines faster and in a more efficient manner than the traditional back crossing and selection strategies. Sunflower is the third most important oilseed crop in the world and has no available double haploid induction procedure/technique that can be efficiently used in breeding programs. A reproducible and efficient doubled haploid induction method would be a valuable tool in accelerating the breeding of new elite sunflower varieties. Although several attempts have been made, the establishment of a sunflower doubled haploid induction protocol has remained a challenge owing recalcitrance to in vitro culture regeneration. Approaches for haploid development in other crops are often cultivar specific, difficult to reproduce, and rely on available tissue culture protocols-which on their own are also cultivar and/or species specific. As an out-crossing crop, the lack of a double haploid system limits sunflower breeding and associated improvement processes, thereby delaying new hybrid and trait developments. Significant molecular advances targeting genes, such as the () and () gene with CRISPR/Cas9, and the successful use of viral vectors for the delivery of CRISPR/Cas9 components into plant cells eliminating the in vitro culture bottleneck, have the potential to improve double haploid technology in sunflower. In this review, the different strategies, their challenges, and opportunities for achieving doubled haploids in sunflower are explored.

摘要

任何育种过程的目标都是在后代中充分表达目标亲本的优良特性。杂交种常用于这个动态且复杂的过程,在此过程中需要纯合亲本,这可能需要多达八代的回交和选择。双单倍体(DH)技术能够比传统的回交和选择策略更快、更高效地培育出纯育品系。向日葵是世界上第三重要的油料作物,目前尚无可有效用于育种计划的双单倍体诱导程序/技术。一种可重复且高效的双单倍体诱导方法将成为加速培育新的优良向日葵品种的宝贵工具。尽管已经进行了多次尝试,但由于向日葵对离体培养再生具有顽拗性,建立向日葵双单倍体诱导方案仍然是一项挑战。其他作物的单倍体发育方法通常具有品种特异性,难以重复,并且依赖于现有的组织培养方案,而这些方案本身也具有品种和/或物种特异性。作为一种异花授粉作物,缺乏双单倍体系统限制了向日葵的育种及相关改良进程,从而延迟了新杂交种和性状的开发。针对基因的重大分子进展,如利用CRISPR/Cas9靶向()和()基因,以及成功使用病毒载体将CRISPR/Cas9组件导入植物细胞以消除离体培养瓶颈,有可能改进向日葵的双单倍体技术。在这篇综述中,我们探讨了在向日葵中实现双单倍体的不同策略、面临的挑战和机遇。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9d2/9921946/d916a1e0abf8/plants-12-00485-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9d2/9921946/6e3d03811a01/plants-12-00485-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9d2/9921946/1193d70d9f90/plants-12-00485-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9d2/9921946/81c088f588b3/plants-12-00485-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9d2/9921946/d916a1e0abf8/plants-12-00485-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9d2/9921946/6e3d03811a01/plants-12-00485-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9d2/9921946/1193d70d9f90/plants-12-00485-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9d2/9921946/81c088f588b3/plants-12-00485-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f9d2/9921946/d916a1e0abf8/plants-12-00485-g004.jpg

相似文献

[1]
Accelerated Breeding for (Sunflower) through Doubled Haploidy: An Insight on Past and Future Prospects in the Era of Genome Editing.

Plants (Basel). 2023-1-20

[2]
Development of a Haploid-Inducer Mediated Genome Editing System for Accelerating Maize Breeding.

Mol Plant. 2019-3-19

[3]
Development and innovation of haploid induction technologies in plants.

Yi Chuan. 2020-5-20

[4]
Haploids: Constraints and opportunities in plant breeding.

Biotechnol Adv. 2015-7-9

[5]
The Production of Haploids: A Review of Its Current Status and Future Prospects.

Plants (Basel). 2022-10-29

[6]
Recent Advances in Engineering of In Vivo Haploid Induction Systems.

Methods Mol Biol. 2023

[7]
Advances in Gene Editing of Haploid Tissues in Crops.

Genes (Basel). 2021-9-13

[8]
MATRILINEAL, a sperm-specific phospholipase, triggers maize haploid induction.

Nature. 2017-1-23

[9]
Developments and prospects for doubled haploid wheat.

Biotechnol Adv. 2022-11

[10]
A variety of changes, including CRISPR/Cas9-mediated deletions, in CENH3 lead to haploid induction on outcrossing.

Plant Biotechnol J. 2020-10

引用本文的文献

[1]
Advancing virus-induced gene silencing in sunflower: key factors of VIGS spreading and a novel simple protocol.

Plant Methods. 2024-8-12

本文引用的文献

[1]
The Production of Haploids: A Review of Its Current Status and Future Prospects.

Plants (Basel). 2022-10-29

[2]
Controlled Induction of Parthenogenesis in Transgenic Rice Post-translational Activation of .

Front Plant Sci. 2022-7-8

[3]
Developments and prospects for doubled haploid wheat.

Biotechnol Adv. 2022-11

[4]
Tools and targets: The dual role of plant viruses in CRISPR-Cas genome editing.

Plant Genome. 2023-6

[5]
Comparative Transcriptome Analysis of Reveals the Molecular Basis for the Recalcitrant Genetic Transformation of L.

Biomolecules. 2022-5-11

[6]
Haploidy and aneuploidy in switchgrass mediated by misexpression of CENH3.

Plant Genome. 2023-6

[7]
Breeding More Crops in Less Time: A Perspective on Speed Breeding.

Biology (Basel). 2022-2-10

[8]
Peculiarities of the Transformation of Family Species: The Cases of Sunflower and Lettuce.

Front Plant Sci. 2021-11-26

[9]
Targeted mutagenesis in plants using Beet curly top virus for efficient delivery of CRISPR/Cas12a components.

N Biotechnol. 2022-3-25

[10]
RNA Interference and CRISPR/Cas Gene Editing for Crop Improvement: Paradigm Shift towards Sustainable Agriculture.

Plants (Basel). 2021-9-14

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