da Silva Andréia Maria, Sousa Patrícia Cunha, Campos Lívia Batista, Bezerra José Artur Brilhante, de Araújo Lago Arthur Emannuel, de Oliveira Moacir Franco, Silva Alexandre Rodrigues
Laboratório de Conservação e Germoplasma Animal,Universidade Federal Rural do Semi-Árido (UFERSA),Mossoró,RN,Brazil.
Departamento de Ciências Animais,Universidade Federal Rural do Semi-Árido (UFERSA),BR 110,Km 47,Bairro Pres. Costa e Silva. CEP: 59.625-900 Mossoró,RN,Brazil.
Zygote. 2017 Apr;25(2):176-182. doi: 10.1017/S0967199417000016. Epub 2017 Mar 1.
The aim of this study was to evaluate the performance of cavy (Galea spixii) epididymal sperm following addition to TES or TRIS extenders and using a thermal resistance test (TRT), as well as fluorescence analysis as a complementary method to predict the viability of these gametes. Nine testicle-epididymis complexes were used for sperm collection using a flotation method. Epididymis tails were sliced and one was immersed in 3 ml of TRIS buffer, and the other in 3 ml of TES, for 5 min. After sperm recovery, the samples were subjected to a TRT which involved incubation in a water bath at 37°C for 3 h. During incubation, sample parameters were assessed at 0, 15, 30, 60, 90, 120, 150 or 180 min intervals. Results indicated that the TRIS diluent was more efficient than TES (P < 0.05) for the maintenance of sperm parameters in Spix's yellow-toothed cavies over the whole TRT, maintaining sperm longevity for an extended time. In conclusion, we indicate the use of TRIS diluent for recovery and maintenance of longevity of epididymal sperm from cavies (G. spixii).
本研究的目的是评估豚鼠（斯氏黄齿天竺鼠）附睾精子添加到TES或TRIS稀释液中后的性能，并使用耐热性试验（TRT）以及荧光分析作为预测这些配子活力的补充方法。使用9个睾丸-附睾复合体通过浮选法收集精子。将附睾尾部切片，一片浸入3毫升TRIS缓冲液中，另一片浸入3毫升TES中，持续5分钟。精子回收后，对样品进行TRT，即将其置于37°C水浴中孵育3小时。在孵育期间，每隔0、15、30、60、90、120、150或180分钟评估样品参数。结果表明，在整个TRT过程中，TRIS稀释液在维持斯氏黄齿天竺鼠精子参数方面比TES更有效（P<0.05），能延长精子寿命。总之，我们表明使用TRIS稀释液来回收和维持豚鼠（斯氏黄齿天竺鼠）附睾精子的寿命。
