Bertrand H A, Anderson W R, Masoro E J, Yu B P
Department of Physiology, University of Texas Health Science Center at San Antonio.
J Gerontol. 1987 Nov;42(6):666-73. doi: 10.1093/geronj/42.6.666.
The effects of aging and of food restriction at different times during life on rat adipocyte responses to glucagon and epinephrine were explored by studying hormone-stimulated lipolysis, hormone binding, and phosphodiesterase activity. The times of food restriction were: (a) from 6 weeks of age, (b) limited to early life, and (c) beginning in young adult life. Hormone-sensitive lipolysis is lost with age. Food restriction from 6 weeks of age prevents this loss, and food restriction started in adult life causes the recovery of this lipolysis. Hormone binding studies reveal that: (a) changes in glucagon-stimulated lipolysis parallel changes in glucagon binding; (b) glucagon binding and glucagon-stimulated lipolysis correlate inversely with cell size; (c) changes in epinephrine-stimulated lipolysis are not due to changes in beta-adrenergic binding; and (d) neither beta-adrenergic binding nor epinephrine-promoted lipolysis correlate with fat cell size. Phosphodiesterase activity is not influenced by diet, making it unlikely to be a postreceptor component lost with age.
通过研究激素刺激的脂肪分解、激素结合和磷酸二酯酶活性，探讨了衰老以及生命不同时期食物限制对大鼠脂肪细胞对胰高血糖素和肾上腺素反应的影响。食物限制的时期为：(a) 从6周龄开始，(b) 限于生命早期，以及(c) 从成年早期开始。激素敏感性脂肪分解随年龄增长而丧失。6周龄开始的食物限制可防止这种丧失，而成年期开始的食物限制可使这种脂肪分解恢复。激素结合研究表明：(a) 胰高血糖素刺激的脂肪分解变化与胰高血糖素结合变化平行；(b) 胰高血糖素结合和胰高血糖素刺激的脂肪分解与细胞大小呈负相关；(c) 肾上腺素刺激的脂肪分解变化并非由于β-肾上腺素能结合的变化；(d) β-肾上腺素能结合和肾上腺素促进的脂肪分解均与脂肪细胞大小无关。磷酸二酯酶活性不受饮食影响，因此不太可能是随年龄丧失的受体后成分。
