在免疫过氧化物酶法中使用叠氮化物和过氧化氢作为内源性过氧化物酶的抑制剂。

Use of azide and hydrogen peroxide as an inhibitor for endogenous peroxidase in the immunoperoxidase method.

作者信息

Li C Y, Ziesmer S C, Lazcano-Villareal O

机构信息

Department of Laboratory Medicine, Mayo Clinic, Rochester, Minnesota 55905.

出版信息

J Histochem Cytochem. 1987 Dec;35(12):1457-60. doi: 10.1177/35.12.2824601.

DOI:10.1177/35.12.2824601

PMID:2824601

Abstract

We describe a simple and effective method for inhibition of endogenous peroxidase activity in the immunoperoxidase technique. Specimens are pre-treated with a mixture of azide and hydrogen peroxide, which is then followed by an indirect immunoperoxidase procedure. Comparison studies showed no significant loss of antigenicity or morphological details by this pre-treatment. The method is most useful for evaluating cell-specific antigens on specimens that have abundant endogenous peroxidase activity, such as blood and bone marrow.

摘要

我们描述了一种在免疫过氧化物酶技术中抑制内源性过氧化物酶活性的简单有效方法。标本先用叠氮化物和过氧化氢的混合物进行预处理，然后进行间接免疫过氧化物酶程序。比较研究表明，这种预处理不会导致抗原性或形态细节的显著损失。该方法对于评估具有丰富内源性过氧化物酶活性的标本（如血液和骨髓）上的细胞特异性抗原最为有用。