通过抗同种型二级抗体对鼠组织上的鼠抗体进行无背景检测。
Background-free Detection of Mouse Antibodies on Mouse Tissue by Anti-isotype Secondary Antibodies.
机构信息
Pathology, Department of Medicine and Surgery, Università di Milano-Bicocca, Monza, Italy.
Department of Veterinary Science, University of Parma, Parma, Italy.
出版信息
J Histochem Cytochem. 2021 Aug;69(8):535-541. doi: 10.1369/00221554211033239. Epub 2021 Jul 20.
Abstract
Immunodetection on mouse routinely processed tissue via antibodies raised in mice faces cross-reactivity of the secondary anti-mouse reagents with endogenous immunoglobulins, which permeate the tissue. Various solutions to this problem have been devised and include endogenous Ig block with anti-mouse Fab fragments or directly conjugated primary antibodies. Mouse isotype-specific antibodies, differently from reagents directed against both heavy and light chains, fail to detect endogenous Ig after fixation and embedding, while providing a clean and specific detection system for mouse antibodies on mouse routinely processed tissue.
摘要
通过在小鼠中产生的抗体对常规处理的小鼠组织进行免疫检测会遇到与组织中渗透的内源性免疫球蛋白发生交叉反应的问题。已经设计了各种解决方案，包括使用抗小鼠 Fab 片段对内源性 Ig 进行阻断或直接连接到初级抗体上。与针对重链和轻链的试剂不同，鼠同种型特异性抗体在固定和包埋后无法检测到内源性 Ig，同时为常规处理的小鼠组织上的小鼠抗体提供了清洁和特异性的检测系统。
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