自动化连续酶联免疫组织化学双重染色法，使用两种 HRP 底物。
Automated sequential chromogenic IHC double staining with two HRP substrates.
机构信息
Assay Teccology, Agilent Technologies, Glostrup, Denmark.
Product Design, Agilent Technologies, Glostrup, Denmark.
出版信息
PLoS One. 2018 Nov 20;13(11):e0207867. doi: 10.1371/journal.pone.0207867. eCollection 2018.
Abstract
Automated IHC double staining using diaminobenzidine and HRP Magenta is illustrated utilizing a new acidic block with sulfuric acid to prevent cross-reactivity. Residual cross-reactivity in double staining is determined to arise from chromogenic-bound antibodies and amplification system during the first part of the double staining.
摘要
使用二氨基联苯胺和 HRP 洋红进行自动化免疫组化双重染色，使用新的含硫酸的酸性阻断剂来防止交叉反应。双重染色中的残留交叉反应被确定是由于在双重染色的第一部分中结合在显色剂上的抗体和放大系统引起的。
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