Caldeira Roberta Lima, Jannotti-Passos Liana Konovaloffi, Dos Santos Carvalho Omar
Laboratório de Helmintologia e Malacologia Médica, Centro de Pesquisas René Rachou/Fiocruz, Av. Augusto de Lima, 1715 Belo Horizonte, MG, Brazil.
Moluscário Lobato Paraense, Centro de Pesquisas René Rachou/Fiocruz, Av. Augusto de Lima, 1715 Belo Horizonte, MG, Brazil.
J Trop Med. 2017;2017:8628971. doi: 10.1155/2017/8628971. Epub 2017 Jan 26.
The low stringency-polymerase chain reaction (LS-PCR) and loop-mediated isothermal amplification (LAMP) assays were used to detect the presence of DNA in (1) Brazilian intermediate hosts (, and ) with patent infections, (2) snails with prepatent infections, (3) various mixtures of infected and noninfected snails; and (4) snails infected with other trematode species. The assays showed high sensitivity and specificity and could detect DNA when one positive snail was included in a pool of 1,000 negative specimens of . These molecular approaches can provide a low-cost, effective, and rapid method for detecting the presence of in pooled samples of field-collected . These assays should aid mapping of transmission sites in endemic areas, especially in low prevalence regions and improve schistosomiasis surveillance. It will be a useful tool to monitor low infection rates of snails in areas where control interventions are leading towards the elimination of schistosomiasis.
采用低严格度聚合酶链反应（LS-PCR）和环介导等温扩增（LAMP）分析法，检测以下样本中DNA的存在情况：（1）患有显性感染的巴西中间宿主（ 、 和 ）；（2）患有隐性感染的蜗牛；（3）感染和未感染蜗牛的各种混合物；以及（4）感染其他吸虫种类的蜗牛。这些分析方法显示出高灵敏度和特异性，当1000份阴性 样本池中包含一只阳性蜗牛时，即可检测到DNA。这些分子方法可为检测野外采集的 混合样本中是否存在 提供一种低成本、有效且快速的方法。这些分析方法应有助于在流行地区，特别是在低流行率地区绘制传播地点图，并改善血吸虫病监测。对于监测控制干预措施正朝着消除血吸虫病方向发展的地区蜗牛的低感染率而言，这将是一个有用的工具。
