温度对重组巨大芽孢杆菌中质粒稳定性及克隆纤维素酶基因表达的影响
Effect of temperature on plasmid stability and expression of cloned cellulase gene in a recombinantBacillus megaterium.
作者信息
Son Kwang H, Jang Jong H, Kim Jung H
机构信息
Department of Biological Science and Engineering, Korea Advanced Institute of Science and Technology, Chongryang, POB 150, 131, Seoul, Korea.
出版信息
Biotechnol Lett. 1987 Dec;9(12):821-824. doi: 10.1007/BF01026189.
The expression of carboxymethyl cellulase gene inBacillus megaterium (pCK108) was investigated with respect to temperature in batch culture. The suboptimal temperature supporting maximal cell growth rate was determined to be 30 °C at which stability of the plasmid pCK108 could be maintained stable. However, cellular plasmid contents, production rate of cellulase of the cell, and efficiency of the gene expression increased significantly with increase of the temperature from 30 °C to 44 °C, even though the plasmid stability decreased up to 60% level at the end of the culture.
在分批培养中，研究了巨大芽孢杆菌（pCK108）中羧甲基纤维素酶基因在不同温度下的表达情况。支持最大细胞生长速率的次优温度被确定为30℃，在此温度下质粒pCK108的稳定性可保持稳定。然而，从30℃升高到44℃时，细胞中的质粒含量、细胞纤维素酶的产生速率以及基因表达效率均显著增加，尽管在培养结束时质粒稳定性下降至60%。
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