Robson L M, Chambliss G H
Appl Environ Microbiol. 1984 May;47(5):1039-46. doi: 10.1128/aem.47.5.1039-1046.1984.
A group I Bacillus strain, DLG, was isolated and characterized as being most closely related to Bacillus subtilis. When grown on any of a variety of sugars, the culture supernatant of this isolate was found to possess cellulolytic activity, as demonstrated by degradation of trinitrophenyl-carboxymethyl cellulose. Growth in medium containing cellobiose or glucose resulted in the greatest production of cellulolytic activity. The cellulolytic activity was not produced until the stationary phase of growth, and the addition of glucose or cellobiose to a culture in this phase had no apparent effect on enzyme production. Fractionation of the culture supernatant showed that the molecular weight of the enzymatic activity was less than 100,000. Maximum cellulolytic activity in assays was observed at pH 4.8 and at 58C, although maximum thermal stability of the activity. Kinetic experiments suggested that more than one enzyme was acting upon trinitrophenyl-carboxymethyl cellulose. Exocellular protein produced by this Bacillus isolate showed roughly one-fifth the cellulolytic activity displayed by Trichoderma reesei C30 on noncrystalline, cellulosic substrates. In contrast to T. reesei cellulase, the Bacillus enzymatic activity showed no ability to degrade crystalline forms of cellulose, nor was cellobiase activity detectable.
分离出了一株I类芽孢杆菌菌株DLG，经鉴定它与枯草芽孢杆菌的亲缘关系最为密切。当在多种糖类中的任何一种上生长时，发现该分离株的培养上清液具有纤维素分解活性，这通过三硝基苯基 - 羧甲基纤维素的降解得以证明。在含有纤维二糖或葡萄糖的培养基中生长导致纤维素分解活性的最大产生。纤维素分解活性直到生长稳定期才产生，在此阶段向培养物中添加葡萄糖或纤维二糖对酶的产生没有明显影响。对培养上清液进行分级分离表明，酶活性的分子量小于100,000。在pH 4.8和58℃下测定时观察到最大纤维素分解活性，尽管该活性的最大热稳定性……动力学实验表明，不止一种酶作用于三硝基苯基 - 羧甲基纤维素。该芽孢杆菌分离株产生的胞外蛋白在非晶态纤维素底物上显示出约为里氏木霉C30所展示的纤维素分解活性的五分之一。与里氏木霉纤维素酶相反，芽孢杆菌的酶活性没有降解结晶形式纤维素的能力，也检测不到纤维二糖酶活性。 （注：原文中“although maximum thermal stability of the activity.”后面似乎缺失内容）
