Ploskonos M V
Department of Chemistry, Astrakhan State Medical University of Minzdrav of Russia.
Department of Natural Sciences, Volga State University of Water Transport of the Caspian Institute of Maritime and River Transport, Astrakhan.
Urologiia. 2016 Aug(4):87-91.
To identify the relationship between phosphatidylserine externalization, as an early marker of apoptosis, and functional and morphological sperm impairment in infertile men to subsequently evaluate the effect of apoptosis on sperm fertility.
Ejaculates of 18 fertile and 78 subfertile men were examined. Phosphatidylserine externalization was detected by staining the sperm with fluorochrome conjugated Annexin V (AnV-FITC) and propidium iodide using fluorescence microscopy.
and conclusions: Ejaculates of fertile and subfertile men differed in the percentage of annexin-V-positive sperm. The correlation of (AnV+/PI+) - sperm of subfertile men with sperm concentration, motility and defects of sperm morphology shows the adverse effects of apoptosis on sperm quality and suggests that phosphatidylserine externalization is a factor for reducing sperm fertility.
确定作为细胞凋亡早期标志物的磷脂酰丝氨酸外化与不育男性精子功能和形态损伤之间的关系，进而评估细胞凋亡对精子生育能力的影响。
对18名有生育能力的男性和78名不育男性的精液进行检查。使用荧光显微镜，通过用荧光染料偶联的膜联蛋白V（AnV-FITC）和碘化丙啶对精子进行染色来检测磷脂酰丝氨酸外化。
有生育能力和不育男性的精液中膜联蛋白-V阳性精子的百分比存在差异。不育男性的（AnV+/PI+）精子与精子浓度、活力及精子形态缺陷之间的相关性表明细胞凋亡对精子质量有不利影响，并提示磷脂酰丝氨酸外化是降低精子生育能力的一个因素。
