Yilmaz Gulsum, Cetin Ender, Bozkurt Umit, Aleksanyan Magden Karin
Department of Environmental Engineering, Istanbul University, Avcilar, Istanbul, 34320, Turkey.
Biotechnol Prog. 2017 May;33(3):716-725. doi: 10.1002/btpr.2456. Epub 2017 Mar 20.
Lab-scale experiments were conducted to investigate the effects of ferrous iron on nutrient removal performance and variations in the microbial community inside aerobic granular sludge for 408 days. Two reactors were simultaneously operated, one without added ferrous iron (SBR1), and one with 10 mg Fe L of added ferrous iron (SBR2). A total of 1 mg Fe L of added ferrous iron was applied to SBR1 starting from the 191st day to observe the resulting variations in the nutrient removal performance and the microbial community. The results show that ammonia-oxidizing bacteria (AOB) could not oxidize ammonia due to a lack of iron compounds, but they could survive in the aerobic granular sludge. Limited ferrous iron addition encouraged nitrification. Enhanced biological phosphorus removal (EBPR) from both reactors could not be maintained regardless of the amount of ferrous iron that was applied. EBPR was established in both reactors when the concentration of mixed liquor suspended solid (MLSS) and the percentage of Accumulibacteria increased. A total of 10 mg Fe L of added ferrous iron had a relatively adverse effect on the growth of AOB species compared to 1 mg Fe L of added ferrous iron, but it encouraged the growth of Nitrospira sp. and Accumulibacteria, which requires further study. It could be said that the compact and stable structure of aerobic granular sludge preserved AOB and NOB from Fe-deficient conditions, and wash-out during the disintegration period. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:716-725, 2017.
进行了实验室规模的实验，以研究亚铁对好氧颗粒污泥内营养物去除性能和微生物群落变化的影响，为期408天。同时运行两个反应器，一个不添加亚铁（SBR1），另一个添加10mg/L的亚铁（SBR2）。从第191天开始，向SBR1中添加总共1mg/L的亚铁，以观察营养物去除性能和微生物群落的相应变化。结果表明，由于缺乏铁化合物，氨氧化细菌（AOB）无法氧化氨，但它们可以在好氧颗粒污泥中存活。添加有限的亚铁促进了硝化作用。无论施加的亚铁量如何，两个反应器中强化生物除磷（EBPR）均无法维持。当混合液悬浮固体（MLSS）浓度和聚磷菌百分比增加时，两个反应器中均建立了EBPR。与添加1mg/L的亚铁相比，添加10mg/L的亚铁对AOB物种的生长具有相对不利的影响，但它促进了硝化螺菌属和聚磷菌的生长，这需要进一步研究。可以说，好氧颗粒污泥的紧凑稳定结构使AOB和硝化菌在缺铁条件下以及解体期间得以保留，避免被冲走。©2017美国化学工程师学会生物技术进展，33:716 - 725，2017。
