Bosman Giel J C G M
Department of Biochemistry (286), Radboud University Medical Center and Radboud Institute for Molecular Sciences, P.O. Box 9101, NL-6500 HB Nijmegen, The Netherlands.
Proteomes. 2016 Nov 25;4(4):35. doi: 10.3390/proteomes4040035.
During the past decade, the hand-in-hand development of biotechnology and bioinformatics has enabled a view of the function of the red blood cell that surpasses the supply of oxygen and removal of carbon dioxide. Comparative proteomic inventories have yielded new clues to the processes that regulate membrane-cytoskeleton interactions in health and disease, and to the ways by which red blood cells communicate with their environment. In addition, proteomic data have revealed the possibility that many, hitherto unsuspected, metabolic processes are active in the red blood cell cytoplasm. Recent metabolomic studies have confirmed and expanded this notion. Taken together, the presently available data point towards the red blood cell membrane as the hub at which all regulatory processes come together. Thus, alterations in the association of regulatory proteins with the cell membrane may be a sine qua non for the functional relevance of any postulated molecular mechanism. From this perspective, comparative proteomics centered on the red blood cell membrane constitute a powerful tool for the identification and elucidation of the physiologically and pathologically relevant pathways that regulate red blood cell homeostasis. Additionally, this perspective provides a focus for the interpretation of metabolomic studies, especially in the development of biomarkers in the blood.
在过去十年中，生物技术与生物信息学携手发展，使人们对红细胞功能的认识超越了氧气供应和二氧化碳清除。比较蛋白质组学研究为健康和疾病状态下调节膜 - 细胞骨架相互作用的过程以及红细胞与周围环境沟通的方式提供了新线索。此外，蛋白质组学数据揭示了许多迄今未被怀疑的代谢过程可能在红细胞细胞质中活跃的可能性。最近的代谢组学研究证实并扩展了这一概念。综合来看，目前可得的数据表明红细胞膜是所有调节过程汇聚的中心。因此，调节蛋白与细胞膜结合的改变可能是任何假定分子机制功能相关性的必要条件。从这个角度来看，以红细胞膜为中心的比较蛋白质组学是识别和阐明调节红细胞稳态的生理和病理相关途径的有力工具。此外，这一观点为代谢组学研究的解释提供了重点，尤其是在血液生物标志物的开发方面。
