Prudinnik Dmitry S, Kussanova Aigul, Vorobjev Ivan A, Tikhonov Alexander, Ataullakhanov Fazly I, Barteneva Natasha S
Department of Biology, School of Sciences and Humanities, Nazarbayev University, Astana 010000, Kazakhstan.
Department of Physiology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Aging Dis. 2024 Jun 19;16(3):1242-1264. doi: 10.14336/AD.2024.0526.
Aging is interrelated with changes in red blood cell parameters and functionality. In this article, we focus on red blood cells (RBCs) and provide a review of the known changes associated with the characterization of RBC deformability in aging and related pathologies. The biophysical parameters complement the commonly used biochemical parameters and may contribute to a better understanding of the aging process. The power of the deformability measurement approach is well established in clinical settings. Measuring RBCs' deformability has the advantage of relative simplicity, and it reflects the complex effects developing in erythrocytes during aging. However, aging and related pathological conditions also promote heterogeneity of RBC features and have a certain impact on the variance in erythrocyte cell properties. The possible applications of deformability as an early biophysical biomarker of pathological states are discussed, and modulating PIEZO1 as a therapeutic target is suggested. The changes in RBCs' shape can serve as a proxy for deformability evaluation, leveraging single-cell analysis with imaging flow cytometry and artificial intelligence algorithms. The characterization of biophysical parameters of RBCs is in progress in humans and will provide a better understanding of the complex dynamics of aging.
衰老与红细胞参数及功能的变化相互关联。在本文中，我们聚焦于红细胞（RBCs），并综述与衰老及相关病理状态下红细胞变形性特征相关的已知变化。生物物理参数补充了常用的生化参数，可能有助于更好地理解衰老过程。变形性测量方法在临床环境中的作用已得到充分确立。测量红细胞的变形性具有相对简单的优点，并且它反映了衰老过程中红细胞内发生的复杂效应。然而，衰老及相关病理状况也会促进红细胞特征的异质性，并对红细胞特性的差异产生一定影响。本文讨论了将变形性作为病理状态早期生物物理生物标志物的可能应用，并提出将PIEZO1作为治疗靶点进行调节。利用成像流式细胞术和人工智能算法进行单细胞分析，红细胞形状的变化可作为变形性评估的替代指标。红细胞生物物理参数的表征在人类研究中正在进行，这将有助于更好地理解衰老的复杂动态过程。
