Li Feng, Li Jingjing, Tang Yanan, Wang Chuan, Li Xing-Fang, Le X Chris
Department of Laboratory Medicine and Pathology, University of Alberta, Edmonton, AB T6G 2G3, Canada.
Proteomes. 2016 Dec 22;5(1):1. doi: 10.3390/proteomes5010001.
The ability to selectively amplify the detection signals for targets over interferences is crucial when analyzing proteins in a complicated sample matrix. Here, we describe a targeted enlargement strategy that can amplify the light-scattering signal from aptamer-functionalized gold nanoparticles (Apt-AuNP) with high specificity for quantitative protein analysis. This strategy is achieved by labeling target proteins with competitively protected Apt-AuNP probes and enlarging the probes with gold enhancement. This competitive protection strategy could effectively eliminate nonspecific protein adsorptions from a sample matrix, leading to a highly specific labeling of the target protein. As a result, the subsequent amplification of the light-scattering signal by gold enhancement only occurs in the presence of the target protein. This strategy was successfully demonstrated by analyzing human α-thrombin in human serum samples in a Western blot format.
在复杂样品基质中分析蛋白质时，选择性放大目标检测信号以对抗干扰的能力至关重要。在此，我们描述了一种靶向放大策略，该策略可对适体功能化金纳米颗粒（Apt-AuNP）的光散射信号进行高特异性放大，用于定量蛋白质分析。此策略通过用竞争性保护的Apt-AuNP探针标记目标蛋白质，并通过金增强来放大探针来实现。这种竞争性保护策略可有效消除样品基质中的非特异性蛋白质吸附，从而实现对目标蛋白质的高特异性标记。因此，随后通过金增强对光散射信号的放大仅在目标蛋白质存在时发生。通过以蛋白质印迹形式分析人血清样品中的人α-凝血酶，成功证明了该策略。
