适体-纳米颗粒条带生物传感器用于灵敏检测癌细胞。
Aptamer-nanoparticle strip biosensor for sensitive detection of cancer cells.
机构信息
Key Laboratory of Regenerative Biology, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, China 510663.
出版信息
Anal Chem. 2009 Dec 15;81(24):10013-8. doi: 10.1021/ac901889s.
Abstract
We report an aptamer-nanoparticle strip biosensor (ANSB) for the rapid, specific, sensitive, and low-cost detection of circulating cancer cells. Known for their high specificity and affinity, aptamers were first selected from live cells by the cell-SELEX (systematic evolution of ligands by exponential enrichment) process. When next combined with the unique optical properties of gold nanoparticles (Au-NPs), ANSBs were prepared on a lateral flow device. Ramos cells were used as a model target cell to demonstrate proof of principle. Under optimal conditions, the ANSB was capable of detecting a minimum of 4000 Ramos cells without instrumentation (visual judgment) and 800 Ramos cells with a portable strip reader within 15 min. Importantly, ANSB has successfully detected Ramos cells in human blood, thus providing a rapid, sensitive, and low-cost quantitative tool for the detection of circulating cancer cells. ANSB therefore shows great promise for in-field and point-of-care cancer diagnosis and therapy.
摘要
我们报告了一种适体-纳米颗粒条带生物传感器（ANSB），用于快速、特异性、敏感和低成本地检测循环癌细胞。适体因其高特异性和亲和力而首先通过细胞 SELEX（指数富集的配体系统进化）过程从活细胞中筛选出来。当与金纳米颗粒（Au-NPs）的独特光学特性结合时，ANSB 被制备在横向流动装置上。Ramos 细胞被用作模型靶细胞来证明原理的可行性。在最佳条件下，ANSB 能够在 15 分钟内无需仪器（目视判断）检测到最低 4000 个 Ramos 细胞，使用便携式条带读取器则可检测到 800 个 Ramos 细胞。重要的是，ANSB 已成功地在人血液中检测到 Ramos 细胞，因此为检测循环癌细胞提供了一种快速、敏感和低成本的定量工具。因此，ANSB 有望用于现场和即时癌症诊断和治疗。
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