Key Laboratory of Regenerative Biology, Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, Guangdong, China 510663.
Anal Chem. 2009 Dec 15;81(24):10013-8. doi: 10.1021/ac901889s.
We report an aptamer-nanoparticle strip biosensor (ANSB) for the rapid, specific, sensitive, and low-cost detection of circulating cancer cells. Known for their high specificity and affinity, aptamers were first selected from live cells by the cell-SELEX (systematic evolution of ligands by exponential enrichment) process. When next combined with the unique optical properties of gold nanoparticles (Au-NPs), ANSBs were prepared on a lateral flow device. Ramos cells were used as a model target cell to demonstrate proof of principle. Under optimal conditions, the ANSB was capable of detecting a minimum of 4000 Ramos cells without instrumentation (visual judgment) and 800 Ramos cells with a portable strip reader within 15 min. Importantly, ANSB has successfully detected Ramos cells in human blood, thus providing a rapid, sensitive, and low-cost quantitative tool for the detection of circulating cancer cells. ANSB therefore shows great promise for in-field and point-of-care cancer diagnosis and therapy.
我们报告了一种适体-纳米颗粒条带生物传感器（ANSB），用于快速、特异性、敏感和低成本地检测循环癌细胞。适体因其高特异性和亲和力而首先通过细胞 SELEX（指数富集的配体系统进化）过程从活细胞中筛选出来。当与金纳米颗粒（Au-NPs）的独特光学特性结合时，ANSB 被制备在横向流动装置上。Ramos 细胞被用作模型靶细胞来证明原理的可行性。在最佳条件下，ANSB 能够在 15 分钟内无需仪器（目视判断）检测到最低 4000 个 Ramos 细胞，使用便携式条带读取器则可检测到 800 个 Ramos 细胞。重要的是，ANSB 已成功地在人血液中检测到 Ramos 细胞，因此为检测循环癌细胞提供了一种快速、敏感和低成本的定量工具。因此，ANSB 有望用于现场和即时癌症诊断和治疗。
