Chowdhury R, Ray A, Ray P, Das J
Biophysics Division, Indian Institute of Chemical Biology, Calcutta.
J Virol. 1987 Dec;61(12):3999-4006. doi: 10.1128/JVI.61.12.3999-4006.1987.
The intercellular replication of the circularly permuted DNA of choleraphage phi 149 involves a concatemeric DNA structure with a size equivalent to six genome lengths. The synthesis of both monomeric and concatemeric DNAs during replication of phi 149 occurred in the cytoplasm. The concatemers served as the substrate for the synthesis of mature phage DNA, which was eventually packaged by a headful mechanism starting from a unique pac site in the concatemeric DNA. Packaging of DNA into phage heads involved binding of concatemeric DNA to the cell membrane. A scheme involving sequential packaging of five headfuls proceeding in the counterclockwise direction from the pac site is proposed. After infection under high-phosphate conditions, the concatemeric DNA intermediates were not formed, although synthesis of monomeric molecules was unaffected.
霍乱噬菌体phi 149的环状置换DNA的细胞内复制涉及一种大小相当于六个基因组长度的串联DNA结构。phi 149复制过程中单体和串联DNA的合成均发生在细胞质中。串联体作为成熟噬菌体DNA合成的底物，最终通过从串联DNA中一个独特的pac位点开始的满头部机制进行包装。DNA包装到噬菌体头部涉及串联DNA与细胞膜的结合。提出了一种从pac位点开始沿逆时针方向依次包装五个满头部的方案。在高磷酸盐条件下感染后，尽管单体分子的合成未受影响，但未形成串联DNA中间体。
