霍乱弧菌噬菌体CP-T1：噬菌体DNA的特性及限制性分析

Guidolin A, Morelli G, Kamke M, Manning P A

J Virol. 1984 Jul;51(1):163-9. doi: 10.1128/JVI.51.1.163-169.1984.

Temperature bacteriophage CP-T1 of Vibrio cholerae has a capsid that is 45 nm in diameter, a contractile tail 65 nm long and 9.5 nm wide, and a baseplate with several spikes or short tail fibers. The linear double-stranded DNA is 43.5 +/- 1.4 kilobases long, and the phage genome is both terminally redundant and partially circularly permuted. The extent of terminal redundancy is ca. 4%, and circular permutation is up to ca. 44%. Circular restriction maps have been constructed for the enzymes HindIII, EcoRI, BamHI, and PstI. By restriction endonuclease and heteroduplex analyses of phage DNA, the presence and location of a site (pac) at which packaging of phage DNA is initiated was established.

霍乱弧菌的温度噬菌体CP-T1有一个直径为45纳米的衣壳、一条65纳米长且9.5纳米宽的收缩尾，以及一个带有多个刺突或短尾丝的基板。线性双链DNA长43.5±1.4千碱基，噬菌体基因组既有末端冗余又有部分环状排列。末端冗余程度约为4%，环状排列高达约44%。已构建了针对HindIII、EcoRI、BamHI和PstI酶的环状限制酶切图谱。通过对噬菌体DNA的限制性内切酶和异源双链分析，确定了噬菌体DNA包装起始位点（pac）的存在和位置。