Tumour Angiogenesis Program, Peter MacCallum Cancer Centre, 305 Grattan St, Melbourne, Victoria 3000, Australia.
Ludwig Institute for Cancer Research, Royal Melbourne Hospital, Parkville, Victoria 3050, Australia.
Sci Data. 2017 Mar 1;4:170009. doi: 10.1038/sdata.2017.9.
Many cell types undergo migration during embryogenesis and disease. Endothelial cells line blood vessels and lymphatics, which migrate during development as part of angiogenesis, lymphangiogenesis and other types of vessel remodelling. These processes are also important in wound healing, cancer metastasis and cardiovascular conditions. However, the molecular control of endothelial cell migration is poorly understood. Here, we present a dataset containing siRNA screens that identify known and novel components of signalling pathways regulating migration of lymphatic endothelial cells. These components are compared to signalling in blood vascular endothelial cells. Further, using high-content microscopy, we captured a dataset of images of migrating cells following transfection with a genome-wide siRNA library. These datasets are suitable for the identification and analysis of genes involved in endothelial cell migration and morphology, and for computational approaches to identify signalling networks controlling the migratory response and integration of cell morphology, gene function and cell signaling. This may facilitate identification of protein targets for therapeutically modulating angiogenesis and lymphangiogenesis in the context of human disease.
许多细胞类型在胚胎发生和疾病过程中发生迁移。内皮细胞排列在血管和淋巴管中，在发育过程中作为血管生成、淋巴管生成和其他类型的血管重塑的一部分而迁移。这些过程在伤口愈合、癌症转移和心血管疾病中也很重要。然而，内皮细胞迁移的分子调控机制了解甚少。在这里，我们提供了一个包含 siRNA 筛选的数据集，该数据集可识别调节淋巴管内皮细胞迁移的信号通路的已知和新组件。将这些组件与血管内皮细胞的信号进行比较。此外，我们使用高内涵显微镜，在转染全基因组 siRNA 文库后捕获了一组迁移细胞的图像数据集。这些数据集适用于鉴定和分析参与内皮细胞迁移和形态的基因，以及用于计算方法来鉴定控制迁移反应和细胞形态、基因功能和细胞信号整合的信号网络。这可能有助于鉴定在人类疾病背景下治疗性调节血管生成和淋巴管生成的蛋白质靶标。
