Cancer Therapeutic Program, The Peter MacCallum Cancer Centre , St Andrews Place, East Melbourne, Victoria 3002, Australia.
Victorian Centre for Functional Genomics, The Peter MacCallum Cancer Centre , St Andrews Place, East Melbourne, Victoria 3002, Australia.
Sci Data. 2014 Jul 8;1:140017. doi: 10.1038/sdata.2014.17. eCollection 2014.
Identification of mechanisms of resistance to histone deacetylase inhibitors, such as vorinostat, is important in order to utilise these anticancer compounds more efficiently in the clinic. Here, we present a dataset containing multiple tiers of stringent siRNA screening for genes that when knocked down conferred sensitivity to vorinostat-induced cell death. We also present data from a miRNA overexpression screen for miRNAs contributing to vorinostat sensitivity. Furthermore, we provide transcriptomic analysis using massively parallel sequencing upon knockdown of 14 validated vorinostat-resistance genes. These datasets are suitable for analysis of genes and miRNAs involved in cell death in the presence and absence of vorinostat as well as computational biology approaches to identify gene regulatory networks.
鉴定对组蛋白去乙酰化酶抑制剂（如伏立诺他）的耐药机制对于在临床上更有效地利用这些抗癌化合物非常重要。在这里，我们提供了一个数据集，其中包含多个层次的严格 siRNA 筛选，用于鉴定敲低后对伏立诺他诱导的细胞死亡敏感的基因。我们还提供了 miRNA 过表达筛选数据，用于鉴定促进伏立诺他敏感性的 miRNA。此外，我们还提供了在敲低 14 个已验证的伏立诺他耐药基因后使用大规模平行测序进行的转录组分析。这些数据集可用于分析在有或没有伏立诺他存在的情况下与细胞死亡相关的基因和 miRNA，以及用于识别基因调控网络的计算生物学方法。
