Radiation Research Division for Industry and Environment, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup-Si, Jeollabuk-Do 56212, Republic of Korea.
Reliability Test Center, Defense Agency for Technology and Quality, Seoul 02455, Republic of Korea.
Sci Total Environ. 2017 Jun 1;587-588:232-239. doi: 10.1016/j.scitotenv.2017.02.126. Epub 2017 Feb 27.
This study examined the use of microbial community structure as a bio-indicator of decomposition levels. High-throughput pyrosequencing technology was used to assess the shift in microbial community of leachate from animal carcass lysimeter. The leachate samples were collected monthly for one year and a total of 164,639 pyrosequencing reads were obtained and used in the taxonomic classification and operational taxonomy units (OTUs) distribution analysis based on sequence similarity. Our results show considerable changes in the phylum-level bacterial composition, suggesting that the microbial community is a sensitive parameter affected by the burial environment. The phylum classification results showed that Proteobacteria (Pseudomonas) were the most influential taxa in earlier decomposition stage whereas Firmicutes (Clostridium, Sporanaerobacter, and Peptostreptococcus) were dominant in later stage under anaerobic conditions. The result of this study can provide useful information on a time series of leachate profiles of microbial community structures and suggest patterns of microbial diversity in livestock burial sites. In addition, this result can be applicable to predict the decomposition stages under clay loam based soil conditions of animal livestock.
本研究考察了微生物群落结构作为分解水平生物指标的应用。高通量焦磷酸测序技术用于评估动物尸体淋滤器中渗滤液微生物群落的变化。在一年的时间里，每月收集渗滤液样本，共获得 164639 条焦磷酸测序reads，并根据序列相似性进行分类和操作分类单元（OTU）分布分析。我们的结果表明，在门水平上细菌组成发生了相当大的变化，这表明微生物群落是受埋葬环境影响的敏感参数。门分类结果表明，在早期分解阶段，变形菌门（假单胞菌）是最具影响力的类群，而在厌氧条件下的后期，厚壁菌门（梭菌、螺旋体菌和消化链球菌）占主导地位。本研究的结果可以为牲畜掩埋场所渗滤液微生物群落结构的时间序列提供有用的信息，并提示微生物多样性的模式。此外，该结果可适用于预测粘土壤土条件下动物牲畜的分解阶段。
