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水果中半乳甘露聚糖组分的药妆功效

Cosmeceutical Effects of Galactomannan Fraction from Fruits .

作者信息

Ali Soegianto

机构信息

Food Technology Program, Faculty of Biotechnology, Atma Jaya Catholic University, Jakarta 12930, Indonesia; Master of Science in Biotechnology Program, Faculty of Biotechnology, Atma Jaya Catholic University, Jakarta 12930, Indonesia.

Master of Science in Biotechnology Program, Faculty of Biotechnology, Atma Jaya Catholic University, Jakarta 12930, Indonesia.

出版信息

Pharmacognosy Res. 2017 Jan-Mar;9(1):39-45. doi: 10.4103/0974-8490.199773.

DOI:10.4103/0974-8490.199773
PMID:28250652
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5330101/
Abstract

BACKGROUND

Cosmeceuticals refer to natural cosmetics with medical-like benefits due to their bioactive contents. Sugar palm fruit () extract has been claimed for its anti-aging effect . However, its active compounds for cosmeceuticals is still unclear.

OBJECTIVE

This study was aimed to extract galactomannan from fruits and test its efficacy for tyrosinase inhibition, antioxidant, and anti-photoaging activities .

MATERIALS AND METHODS

Galactomannan from fruits was extracted by freeze drying and identified for its chemical compounds by using pyrolysis gas chromatography-mass spectrometry (py-GC/MS). Galactomannan was tested for its tyrosinase inhibition in both cell-based (melanocytes) and enzymatic assays, antioxidant activity using ferrous ion chelating assay (FCA) assay, and anti-photoaging activity for inhibiting the gene expression of matrix metalloproteinase-1 (MMP-1) and MMP-13 in macrophages using quantitative real-time polymerase chain reaction (qRT-PCR) analysis.

RESULTS

Identification of galactomannan fraction from fruits by py-GC/MS mainly consisted of oxonium ion and glucosides. For cellular assay, galactomannan at 5 μg/mL inhibited >50% of tyrosinase activity in melanocytes induced by phorbol myristate acetate. At the enzymatic level, galactomannan at similar concentration showed less tyrosinase activity inhibition (~20%). FCA results showed that galactomannan at 10 μg/mL exerted >50% of antioxidant activity. The qRT-PCR data indicated that galactomannan at 5 μg/mL inhibited >50% of MMP-1 and MMP-13 gene expressions in ultraviolet B-treated macrophages.

CONCLUSION

Galactomannan fraction from fruits has efficacy for enlightening effect, antioxidant, and anti-photoaging activity in the dose-independent pattern, indicating its cosmeceutical effects for skin healthcare.

SUMMARY

fruit containing galactomannan has cosmeceutical potentials through enlightening effect, antioxidant, and anti-photoaging activity in vitro.Galactomannan fraction has inhibitory effect on tyrosinase activity in both cellular melanocytes and enzymatic systems.Galactomannan fraction has strong protection against UVB-irradiation effect by inhibiting collagenase genes (MMP-1 and MMP-13) in macrophages. : Py-GC/MS: Pyrolysis-Gas Chromatography-Mass Spectrometry; FCA: Ferrous chelating activity; MMP: Matrix metalloproteinase; qRT-PCR: Quantitative Real-Time Polymerase Chain Reaction; PMA: Phorbol myristate acetate; UV: Ultraviolet; RPMI: Roswell Park Memorial Institute; DMEM: Dulbecco's modified eagle media; FBS: Fetal bovine serum; PBS: Phosphate buffered saline; MTT: 3-(4,5-diethylthiazol-2-yl)-2,5-dipheniltetrazolium bromide; L-DOPA: L-3,4-dihydroxyphenylalanine; EDTA: Ethylenediaminetetraacetic acid; GAPDH: Glyceraldehyde 3-phosphate dehydrogenase; DPPH: 1,1-diphenyl-2-picryl-hydrazyl; SPF: Sun protection factor.

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba9/5330101/c73fc139b010/PR-9-39-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba9/5330101/6b0f76f18521/PR-9-39-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba9/5330101/765f4c6b3dff/PR-9-39-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba9/5330101/95c61b56cad1/PR-9-39-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba9/5330101/4b947544ddb5/PR-9-39-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba9/5330101/1843911e12f5/PR-9-39-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba9/5330101/c73fc139b010/PR-9-39-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba9/5330101/6b0f76f18521/PR-9-39-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba9/5330101/765f4c6b3dff/PR-9-39-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba9/5330101/95c61b56cad1/PR-9-39-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba9/5330101/4b947544ddb5/PR-9-39-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba9/5330101/1843911e12f5/PR-9-39-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ba9/5330101/c73fc139b010/PR-9-39-g009.jpg
摘要

背景

药妆品是指因其生物活性成分而具有类似医学功效的天然化妆品。糖棕果提取物据称具有抗衰老作用。然而，其作为药妆品的活性成分仍不清楚。

目的

本研究旨在从糖棕果中提取半乳甘露聚糖，并测试其对酪氨酸酶的抑制、抗氧化和抗光老化活性。

材料与方法

通过冷冻干燥从糖棕果中提取半乳甘露聚糖，并使用热解气相色谱-质谱联用仪（py-GC/MS）鉴定其化学成分。在基于细胞（黑素细胞）和酶促试验中测试半乳甘露聚糖对酪氨酸酶的抑制作用，使用亚铁离子螯合试验（FCA）测定抗氧化活性，并使用定量实时聚合酶链反应（qRT-PCR）分析在巨噬细胞中抑制基质金属蛋白酶-1（MMP-1）和MMP-13基因表达的抗光老化活性。

结果

通过py-GC/MS鉴定糖棕果中的半乳甘露聚糖部分主要由氧鎓离子和糖苷组成。在细胞试验中，5μg/mL的半乳甘露聚糖抑制了佛波酯诱导的黑素细胞中>50%的酪氨酸酶活性。在酶水平上，相似浓度的半乳甘露聚糖显示出较低的酪氨酸酶活性抑制率（约20%）。FCA结果表明，10μg/mL的半乳甘露聚糖具有>50%的抗氧化活性。qRT-PCR数据表明，5μg/mL的半乳甘露聚糖抑制了紫外线B处理的巨噬细胞中>50%的MMP-1和MMP-13基因表达。

结论

糖棕果中的半乳甘露聚糖部分具有美白、抗氧化和抗光老化活性，且呈剂量非依赖性，表明其对皮肤保健具有药妆功效。

总结

含有半乳甘露聚糖的糖棕果通过体外美白、抗氧化和抗光老化活性具有药妆潜力。半乳甘露聚糖部分对细胞黑素细胞和酶系统中的酪氨酸酶活性均有抑制作用。半乳甘露聚糖部分通过抑制巨噬细胞中的胶原酶基因（MMP-1和MMP-13）对紫外线B辐射效应具有强大的保护作用。：py-GC/MS：热解气相色谱-质谱联用仪；FCA：亚铁螯合活性；MMP：基质金属蛋白酶；qRT-PCR：定量实时聚合酶链反应；PMA：佛波酯；UV：紫外线；RPMI：罗斯威尔公园纪念研究所；DMEM：杜尔贝科改良伊格尔培养基；FBS：胎牛血清；PBS：磷酸盐缓冲盐水；MTT：3-(4,5-二乙基噻唑-2-基)-2,5-二苯基四氮唑溴盐；L-DOPA：L-3,4-二羟基苯丙氨酸；EDTA：乙二胺四乙酸；GAPDH：甘油醛-3-磷酸脱氢酶；DPPH：1,1-二苯基-2-苦基肼；SPF：防晒系数

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