Efimova M A, Shah Mahmud R, Zelenikhin P V, Sabirova M I, Kolpakov A I, Ilinskaya O N
Federal Center for Toxicological, Radiation and Biological Safety, Kazan, 420075 Russia.
Institute of Fundamental Medicine and Biology, Kazan (Volga Region) Federal University, Kazan, 420008 Russia.
Mol Biol (Mosk). 2017 Jan-Feb;51(1):111-117. doi: 10.7868/S0026898416060045.
The experimental study identified the antiviral activity of Bacillus pumilus RNase (binase) against the reovirus of serotype 1/strain Lang. For the first time, it has been found that 50 μg/mL of binase effectively reduced the hemagglutinin and cytocidal activity of reovirus in Vero cell line. The preincubation of the enzyme with reovirus before infection of the cells inhibited the viral replication. To determine the stagedependent effect of reovirus reproduction upon binase inhibition, the infected cells were treated with binase or RNase A at different phases of the infectious cycle. The treatment of virus-infected cells has revealed that both enzymes have a maximal antiviral effect on the reovirus propagation during early phases of the reovirus reproduction cycle, with binase being more effective than RNase A. It has been hypothesized that the combined action of the oncolytic reovirus and binase is promising for the elimination of tumor cells carrying mutated RAS gene.
实验研究确定了短小芽孢杆菌核糖核酸酶（binase）对1型/朗株呼肠孤病毒的抗病毒活性。首次发现，50μg/mL的binase能有效降低呼肠孤病毒在Vero细胞系中的血凝素和杀细胞活性。在细胞感染前将该酶与呼肠孤病毒预孵育可抑制病毒复制。为了确定呼肠孤病毒繁殖对binase抑制的阶段依赖性影响，在感染周期的不同阶段用binase或核糖核酸酶A处理感染的细胞。对病毒感染细胞的处理表明，这两种酶在呼肠孤病毒繁殖周期的早期阶段对呼肠孤病毒的传播具有最大的抗病毒作用，其中binase比核糖核酸酶A更有效。据推测，溶瘤呼肠孤病毒和binase的联合作用有望消除携带突变RAS基因的肿瘤细胞。
