Silva A V C, Nascimento A L S, Vitória M F, Rabbani A R C, Soares A N R, Lédo A S
Embrapa Tabuleiros Costeiros, Aracaju, SE, Brasil
Embrapa Tabuleiros Costeiros, Aracaju, SE, Brasil.
Genet Mol Res. 2017 Feb 23;16(1):gmr-16-01-gmr.16019402. doi: 10.4238/gmr16019402.
Banana (Musa spp) is a fruit species frequently cultivated and consumed worldwide. Molecular markers are important for estimating genetic diversity in germplasm and between genotypes in breeding programs. The objective of this study was to analyze the genetic diversity of 21 banana genotypes (FHIA 23, PA42-44, Maçã, Pacovan Ken, Bucaneiro, YB42-47, Grand Naine, Tropical, FHIA 18, PA94-01, YB42-17, Enxerto, Japira, Pacovã, Prata-Anã, Maravilha, PV79-34, Caipira, Princesa, Garantida, and Thap Maeo), by using inter-simple sequence repeat (ISSR) markers. Material was generated from the banana breeding program of Embrapa Cassava & Fruits and evaluated at Embrapa Coastal Tablelands. The 12 primers used in this study generated 97.5% polymorphism. Four clusters were identified among the different genotypes studied, and the sum of the first two principal components was 48.91%. From the Unweighted Pair Group Method using Arithmetic averages (UPGMA) dendrogram, it was possible to identify two main clusters and subclusters. Two genotypes (Garantida and Thap Maeo) remained isolated from the others, both in the UPGMA clustering and in the principal cordinate analysis (PCoA). Using ISSR markers, we could analyze the genetic diversity of the studied material and state that these markers were efficient at detecting sufficient polymorphism to estimate the genetic variability in banana genotypes.
香蕉（芭蕉属）是一种在全球广泛种植和食用的水果品种。分子标记对于评估种质资源的遗传多样性以及育种计划中不同基因型之间的遗传多样性非常重要。本研究的目的是通过使用简单序列重复区间（ISSR）标记来分析21种香蕉基因型（FHIA 23、PA42 - 44、Maçã、Pacovan Ken、Bucaneiro、YB42 - 47、大麦克、热带、FHIA 18、PA94 - 01、YB42 - 17、Enxerto、Japira、Pacovã、Prata - Anã、Maravilha、PV79 - 34、Caipira、Princesa、Garantida和Thap Maeo）的遗传多样性。材料来自巴西农牧业研究公司木薯与水果研究所的香蕉育种计划，并在巴西农牧业研究公司海岸高原研究所进行评估。本研究中使用的12条引物产生了97.5%的多态性。在所研究的不同基因型中鉴定出四个聚类，前两个主成分的总和为48.91%。从基于算术平均数的非加权配对组方法（UPGMA）聚类图中，可以识别出两个主要聚类和子聚类。在UPGMA聚类和主坐标分析（PCoA）中，有两个基因型（Garantida和Thap Maeo）与其他基因型保持隔离。使用ISSR标记，我们可以分析所研究材料的遗传多样性，并表明这些标记在检测足够的多态性以估计香蕉基因型的遗传变异性方面是有效的。
