利用ISSR标记对重要药用园艺作物枸橼进行遗传多样性分析。

Genetic Diversity Analysis of Medicinally Important Horticultural Crop Aegle marmelos by ISSR Markers.

作者信息

Mujeeb Farina, Bajpai Preeti, Pathak Neelam, Verma Smita Rastogi

机构信息

Department of Biosciences, Integral University, Lucknow, 226026, India.

Department of Biotechnology, Delhi Technological University, Delhi, 110042, India.

出版信息

Methods Mol Biol. 2017;1620:195-211. doi: 10.1007/978-1-4939-7060-5_14.

DOI:10.1007/978-1-4939-7060-5_14

PMID:28540710

Abstract

Inter simple sequence repeat (ISSR) markers help in identifying and determining the extent of genetic diversity in cultivars. Here, we describe their application in determining the genetic diversity of bael (Aegle marmelos Corr.). Universal ISSR primers are selected and their marker characteristics such as polymorphism information content, effective multiplex ratio and marker index have been evaluated. ISSR-PCR is then performed using universal ISSR primers to generate polymorphic bands. This information is used to determine the degree of genetic similarity among the bael varieties/accessions by cluster analysis using unweighted pair-group method with arithmetic averages (UPGMA). This technology is valuable for biodiversity conservation and for making an efficient choice of parents in breeding programs.

摘要

简单重复序列区间（ISSR）标记有助于识别和确定栽培品种的遗传多样性程度。在此，我们描述了它们在确定印度枳椇（Aegle marmelos Corr.）遗传多样性中的应用。选择了通用ISSR引物，并评估了它们的标记特征，如多态性信息含量、有效多重比率和标记指数。然后使用通用ISSR引物进行ISSR-PCR以产生多态性条带。通过使用算术平均数的非加权配对组方法（UPGMA）进行聚类分析，利用这些信息来确定印度枳椇品种/种质间的遗传相似程度。这项技术对于生物多样性保护以及在育种计划中高效选择亲本具有重要价值。