Klotman M E, Henry S C, Hamilton J D
Veterans Administration Medical Center, Durham, North Carolina 27705.
Transplantation. 1987 Nov;44(5):636-9. doi: 10.1097/00007890-198711000-00008.
Cytomegalovirus is present in a latent state in renal allografts and may be reactivated in recipients. While human and murine strains are alike in that a primary infection occurs in seronegative recipients of a kidney from a seropositive donor, they may differ when the recipient is seropositive. In a murine transplant model, superinfection of a seropositive recipient with a second strain is unusual. Reports in human transplantation indicate that superinfection of a seropositive recipient does occur, however the frequency is unknown. Our studies examine the potential importance of specific viral strains in host and recipient, the contribution of prior humoral immunity in the recipient, and the technical ability to identify distinctive strains of virus in the presence of each other. Our results indicate that reversal of the viral strains in donor or recipient animals does not alter our previous observation that reactivation of the endogenous recipient viral strain predominates as the infecting strain in the posttransplant period. Further, the presence of antibody in nearly all donors and recipients confirms that all animals were originally infected prior to transplantation. Finally, we demonstrated the technical ability to detect one virus in the presence of the other, thus excluding this variable as possibly confounding. We conclude that the endogenous, latent recipient strain of cytomegalovirus in the murine model is preferentially reactivated in the posttransplant interval.
巨细胞病毒以潜伏状态存在于同种异体肾移植中，可能在受者体内重新激活。虽然人类和鼠类毒株有相似之处，即血清学阴性的受者接受来自血清学阳性供者的肾脏时会发生原发性感染，但当受者为血清学阳性时，它们可能有所不同。在鼠类移植模型中，血清学阳性的受者被第二种毒株重复感染的情况并不常见。人类移植的报告表明，血清学阳性的受者确实会发生重复感染，但其频率尚不清楚。我们的研究探讨了特定病毒毒株在宿主和受者中的潜在重要性、受者先前体液免疫的作用，以及在相互存在的情况下识别不同病毒毒株的技术能力。我们的结果表明，供体或受体动物中病毒毒株的逆转并不改变我们之前的观察结果，即内源性受体病毒毒株的重新激活在移植后时期作为感染毒株占主导地位。此外，几乎所有供体和受体中都存在抗体，这证实所有动物在移植前最初都受到了感染。最后，我们展示了在另一种病毒存在的情况下检测一种病毒的技术能力，从而排除了这个可能造成混淆的变量。我们得出结论，在鼠类模型中，内源性潜伏的受体巨细胞病毒毒株在移植后的间隔期优先被重新激活。
