Tahir Haroon Elrasheid, Xiaobo Zou, Zhihua Li, Jiyong Shi, Zhai Xiaodong, Wang Sheng, Mariod Abdalbasit Adam
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Rd., 212013 Zhenjiang, Jiangsu, China.
Food Chem. 2017 Jul 1;226:202-211. doi: 10.1016/j.foodchem.2017.01.024. Epub 2017 Jan 7.
Fourier transform infrared with attenuated total reflectance (FTIR-ATR) and Raman spectroscopy combined with partial least square regression (PLSR) were applied for the prediction of phenolic compounds and antioxidant activity in honey. Standards of catechin, syringic, vanillic, and chlorogenic acids were used for the identification and quantification of the individual phenolic compounds in six honey varieties using HPLC-DAD. Total antioxidant activity (TAC) and ferrous chelating capacity were measured spectrophotometrically. For the establishment of PLSR model, Raman spectra with Savitzky-Golay smoothing in wavenumber region 1500-400cm was used while for FTIR-ATR the wavenumber regions of 1800-700 and 3000-2800cm with multiplicative scattering correction (MSC) and Savitzky-Golay smoothing were used. The determination coefficients (R) were ranged from 0.9272 to 0.9992 for Raman while from 0.9461 to 0.9988 for FTIT-ART. The FTIR-ATR and Raman demonstrated to be simple, rapid and nondestructive methods to quantify phenolic compounds and antioxidant activities in honey.
采用傅里叶变换红外衰减全反射光谱（FTIR-ATR）和拉曼光谱结合偏最小二乘回归（PLSR）对蜂蜜中的酚类化合物和抗氧化活性进行预测。使用儿茶素、丁香酸、香草酸和绿原酸标准品，通过高效液相色谱-二极管阵列检测法（HPLC-DAD）对六个蜂蜜品种中的单个酚类化合物进行鉴定和定量。采用分光光度法测定总抗氧化活性（TAC）和亚铁螯合能力。在建立PLSR模型时，使用在波数范围1500-400cm进行Savitzky-Golay平滑处理的拉曼光谱，而对于FTIR-ATR，则使用在1800-700cm和3000-2800cm波数范围进行多元散射校正（MSC）和Savitzky-Golay平滑处理的光谱。拉曼光谱的测定系数（R）范围为0.9272至0.9992，而FTIT-ART的测定系数范围为0.9461至0.9988。FTIR-ATR和拉曼光谱被证明是定量蜂蜜中酚类化合物和抗氧化活性的简单、快速且无损的方法。
