Department of Pharmaceutical Chemistry, College of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa.
Department of Pharmaceutical Chemistry, College of Health Sciences, University of KwaZulu-Natal, Durban 4000, South Africa.
Mater Sci Eng C Mater Biol Appl. 2017 May 1;74:27-35. doi: 10.1016/j.msec.2016.12.126. Epub 2017 Jan 7.
A gold nanourchins modified glassy carbon electrode (AuNu/GCE) was developed for the determination of antimalarial drug, primaquine (PQ). The surface of AuNu/GCE was characterized by electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and cyclic voltammetry (CV). EIS results indicated that the electron transfer process at AuNu/GCE was faster as compared to the bare electrode. The SEM and TEM image confirmed the presence and uniform dispersion of gold nanourchins on the GCE surface. Upon investigating the electrochemical behavior of PQ at AuNu/GCE, the developed sensor was found to exhibit high electrocatalytic activity towards the oxidation of PQ. Under optimal experimental conditions, the sensor showed fast and sensitive current response to PQ over a linear concentration range of 0.01-1μM and 0.001-1μM with a detection limit of 3.5nM and 0.9nM using differential pulse voltammetry (DPV) and square wave voltammetry (SWV), respectively. The AuNu/GCE showed good selectivity, reproducibility and stability. Further, the developed sensor was successfully applied to determine the drug in human urine samples and pharmaceutical formulations demonstrating its analytical applicability in clinical analysis as well as quality control. The proposed method thus provides a promising alternative in routine sensing of PQ as well as promotes the application of gold nanourchins in electrochemical sensors.
一种金纳米棒修饰玻碳电极（AuNu/GCE）被开发用于抗疟药物伯氨喹（PQ）的测定。AuNu/GCE 的表面通过电化学阻抗谱（EIS）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）和循环伏安法（CV）进行了表征。EIS 结果表明，与裸电极相比，AuNu/GCE 上的电子转移过程更快。SEM 和 TEM 图像证实了金纳米棒在 GCE 表面的存在和均匀分散。在研究 AuNu/GCE 上 PQ 的电化学行为时，发现该传感器对 PQ 的氧化具有高电催化活性。在最佳实验条件下，该传感器在差分脉冲伏安法（DPV）和方波伏安法（SWV）下，对 PQ 在 0.01-1μM 和 0.001-1μM 的线性浓度范围内表现出快速和灵敏的电流响应，检测限分别为 3.5nM 和 0.9nM。AuNu/GCE 表现出良好的选择性、重现性和稳定性。此外，该传感器成功地应用于测定人尿样和药物制剂中的药物，证明了其在临床分析和质量控制中的分析适用性。因此，该方法为常规检测 PQ 提供了一种有前途的替代方法，并促进了金纳米棒在电化学传感器中的应用。
