Bera Kaushik, Ray Sayani, Raja Washim, Ray Bimalendu
Natural Products Laboratory, Department of Chemistry, Golapbag, Burdwan, The University of Burdwan, West Bengal 713 104, India.
Natural Products Laboratory, Department of Chemistry, Golapbag, Burdwan, The University of Burdwan, West Bengal 713 104, India.
Int J Biol Macromol. 2017 Jun;99:300-307. doi: 10.1016/j.ijbiomac.2017.02.068. Epub 2017 Feb 28.
The complication of arabinogalactan protein (AGP) structure, a significant ingredient of gum polysaccharides, not merely hinders the allocation of its role, but restricts its utilization as well. Here, we describe structural details of an AGP purified from Aegle marmelos fruit gum. This AGP (310×10g/mol), which is water-soluble, contains β-1,3-linked galactopyranosyl main chain substituted at O-6 position with side chains containing galactose and arabinose residues. Also data on sugar composition, ring size, glycosidic linkage pattern, anomeric configuration and sequence of monosaccharide units of a number of oligosaccharides produced from this AGP by chemical and enzymatic methods were acquired. Biochemical analysis reveals resemblance in antioxidative potential between this arabinogalactan protein and standard antioxidants. Moreover, mixture of AGP and β-lactoglobulin form stable water soluble complex having binding constant K=2.38×10/M. As gum polysaccharides are important raw materials of food industry discovering an antioxidative gum with the ability of creating stable water soluble complex with β-lactoglobulin may have important implication.
阿拉伯半乳聚糖蛋白（AGP）是树胶多糖的一种重要成分，其结构的复杂性不仅阻碍了其功能的发挥，也限制了其应用。在此，我们描述了从木苹果果实树胶中纯化得到的一种AGP的结构细节。这种AGP（310×10g/mol）可溶于水，含有β-1,3-连接的吡喃半乳糖基主链，在O-6位被含有半乳糖和阿拉伯糖残基的侧链取代。此外，还获得了通过化学和酶法从该AGP产生的多种寡糖的糖组成、环大小、糖苷键模式、异头构型和单糖单元序列的数据。生化分析表明，这种阿拉伯半乳聚糖蛋白与标准抗氧化剂在抗氧化潜力上具有相似性。此外，AGP和β-乳球蛋白的混合物形成了稳定的水溶性复合物，结合常数K=2.38×10/M。由于树胶多糖是食品工业的重要原料，发现一种具有抗氧化能力且能与β-乳球蛋白形成稳定水溶性复合物的树胶可能具有重要意义。
