Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian, People's Republic of China.
J Agric Food Chem. 2012 Sep 19;60(37):9424-9. doi: 10.1021/jf302619c. Epub 2012 Sep 6.
A water-soluble arabinogalactan-protein (AGP), designated LRGP3, was isolated from the fruits of Lycium ruthenicum Murr. Its average molecular weight was 75.6 kDa. Monosaccharide composition analysis revealed that it was composed of rhamnose, arabinose, and galactose in a molar ratio of 1.0:14.9:10.4. Protein accounted for 1.7% of the AGP and was rich in hydroxyproline. On the basis of monosaccharide composition, partial acid hydrolysis, methylation analysis, ESI-MS, and NMR spectroscopy, LRGP3 was characterized as a highly branched polysaccharide with a backbone of (1→3)-linked β-D-galactopyranosyl residues, many of which were substituted at the O-6 position by galactosyl or arabinosyl groups. The branches were composed of (1→5)-linked arabinose, (1→2)-linked arabinose, (1→6)-linked galactose, (1→3)-linked galactose, and (1→2,4)-linked rhamnose, and the major nonreducing termini were α-L-arabinofuranosyl residues.
从黑果枸杞果实中分离得到一种水溶性阿拉伯半乳聚糖蛋白（AGP），命名为 LRGP3。其平均分子量为 75.6 kDa。单糖组成分析表明，它由鼠李糖、阿拉伯糖和半乳糖组成，摩尔比为 1.0:14.9:10.4。AGP 中蛋白含量为 1.7%，且富含羟脯氨酸。根据单糖组成、部分酸水解、甲基化分析、ESI-MS 和 NMR 光谱，LRGP3 被鉴定为具有高度分支的多糖，其主链由（1→3）-连接的β-D-半乳糖吡喃基残基组成，其中许多残基在 O-6 位被半乳糖基或阿拉伯糖基取代。支链由（1→5）-连接的阿拉伯糖、（1→2）-连接的阿拉伯糖、（1→6）-连接的半乳糖、（1→3）-连接的半乳糖和（1→2,4）-连接的鼠李糖组成，主要非还原末端为α-L-阿拉伯呋喃糖基残基。
