Xiang Haiyan, Fan Dengxin, Li Huan, Shi Shuyun
School of Pharmaceutical Sciences, Southern Medical University, Guangzhou 510515, PR China; College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, PR China.
J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Apr 1;1049-1050:1-7. doi: 10.1016/j.jchromb.2017.02.014. Epub 2017 Feb 14.
Rapid and selective extraction of target compounds from complex matrices was significantly important for their quantification. Here, hollow porous molecular imprinted polymers (HPMIPs) were prepared using cinnamic acid (CMA) as template, 4-vinylpyridine (4-VP) as functional monomer, and MCM-48 as sacrificial support. Hollow porous structure with large surface area (534.1m/g) made most recognition sites locate on the surface of HPMIPs, resulting in high binding capacity (14.84mg/g) and fast kinetic binding (40min). In comparison, binding capacity and binding equilibrium time for solid MIPs were 4.01mg/g and 16h. Equilibrium data fitted well to Freundlich equation, and adsorption process with multi-diffusion mechanisms could be described by pseudo-second order model. In addition, HPMIPs exhibited favorable selectivity. Finally, HPMIPs were successfully used as a molecular sorbent to efficiently and selectively extract CMA from apple and orange juices. Coupling with high-performance liquid chromatography and UV detection (HPLC-UV), the limit of detection for CMA was 0.006mg/L, and the recoveries (89.3-103.5%) with RSD values ranged from 4.3% to 8.0% were relatively satisfactory. Results indicated the superiority of HPMIPs in the selective extraction field.
从复杂基质中快速、选择性地提取目标化合物对其定量分析至关重要。在此，以肉桂酸（CMA）为模板、4-乙烯基吡啶（4-VP）为功能单体、MCM-48为牺牲载体，制备了中空多孔分子印迹聚合物（HPMIPs）。具有大表面积（534.1m²/g）的中空多孔结构使大多数识别位点位于HPMIPs表面，导致其具有高结合容量（14.84mg/g）和快速动力学结合（40分钟）。相比之下，固体MIPs的结合容量和结合平衡时间分别为4.01mg/g和16小时。平衡数据很好地拟合了Freundlich方程，具有多扩散机制的吸附过程可用伪二级模型描述。此外，HPMIPs表现出良好的选择性。最后，HPMIPs成功用作分子吸附剂，从苹果汁和橙汁中高效、选择性地提取CMA。与高效液相色谱和紫外检测（HPLC-UV）联用，CMA的检测限为0.006mg/L，回收率（89.3 - 103.5%）相对令人满意，相对标准偏差（RSD）值在4.3%至8.0%之间。结果表明HPMIPs在选择性提取领域的优越性。
