Tippabathani Jayakrishna, Nellore Jayshree, Radhakrishnan Vaishnavie, Banik Somashree, Kapoor Sonia
Department of Biotechnology, Sathyabama University, Chennai 600119, India.
Parkinsons Dis. 2017;2017:6025358. doi: 10.1155/2017/6025358. Epub 2017 Jan 31.
Here, we study the expression of NURR1 and FOXA1 mRNA in peripheral blood lymphocytes and its haplotypes in coding region in a small Chennai population of India. Thirty cases of Parkinson's patients (PD) with anti-PD medications (20 males aged 65.85 ± 1.19 and 10 females aged 65.7 ± 1.202) and 30 age matched healthy people (20 males aged 68.45 ± 1.282 and 10 females aged 65.8 ± 1.133) were included. The expression of NURR1 and FOXA1 in PBL was detected by Q-PCR and haplotypes were identified by PCR-SSCP. In the 30 PD cases examined, NURR1 and FOXA1 expression was significantly reduced in both male and female PD patients. However, NURR1 (57.631% reduced in males; 28.93% in females) and FOXA1 (64.42% in males; 55.76% in females) mRNA expression did differ greatly between male and female PD patients. Polymorphisms were identified at exon 4 of the NURR1 and at exon 3 of the FOXA1, respectively, in both male and female patients. A near significant difference in SSCP patterns between genders of control and PD population was analyzed suggesting that further investigations of more patients, more molecular markers, and coding regions should be performed. Such studies could potentially reveal peripheral molecular marker of early PD and different significance to the respective genders.
在此，我们研究了印度钦奈一小部分人群外周血淋巴细胞中NURR1和FOXA1 mRNA的表达及其编码区的单倍型。纳入了30例正在服用抗帕金森病药物的帕金森病患者（PD）（20名男性，年龄65.85±1.19岁；10名女性，年龄65.7±1.202岁）和30名年龄匹配的健康人（20名男性，年龄68.45±1.282岁；10名女性，年龄65.8±1.133岁）。通过Q-PCR检测外周血淋巴细胞中NURR1和FOXA1的表达，并通过PCR-SSCP鉴定单倍型。在所检测的30例PD患者中，男性和女性PD患者的NURR1和FOXA1表达均显著降低。然而，男性和女性PD患者的NURR1（男性降低57.631%；女性降低28.93%）和FOXA1（男性降低64.42%；女性降低55.76%）mRNA表达确实存在很大差异。在男性和女性患者中，分别在NURR1的第4外显子和FOXA1的第3外显子处鉴定到多态性。分析了对照组和PD组人群性别之间SSCP模式的近显著差异，表明应进一步对更多患者、更多分子标记和编码区进行研究。此类研究可能会揭示早期PD的外周分子标记以及对不同性别的不同意义。
