Calleja Enrique, Alarcón Balbino, Oeste Clara L
Cell Biology and Immunology Department, Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas, Universidad Autónoma de Madrid, Madrid, Spain.
Methods Mol Biol. 2017;1584:89-99. doi: 10.1007/978-1-4939-6881-7_7.
Establishing a stable interaction between a T cell and an antigen presenting cell (APC) involves the formation of an immune synapse (IS). It is through this structure that the T cell can integrate all the signals provided by the APC. The IS also serves as a mechanism for TCR downregulation through internalization. Here, we describe methods for visualizing MHC-engaged T cell receptor (TCR) internalization from the IS in human cell lines and mouse primary T cells by confocal fluorescence microscopy techniques.
在T细胞与抗原呈递细胞（APC）之间建立稳定的相互作用涉及免疫突触（IS）的形成。正是通过这种结构，T细胞能够整合APC提供的所有信号。免疫突触也是通过内化作用下调T细胞受体（TCR）的一种机制。在这里，我们描述了通过共聚焦荧光显微镜技术在人细胞系和小鼠原代T细胞中观察从免疫突触内化的MHC结合T细胞受体（TCR）的方法。
