Georgiou C D, Webster D A
Department of Biology, Illinois Institute of Technology, Chicago 60616.
Arch Microbiol. 1987 Oct;148(4):328-33. doi: 10.1007/BF00456712.
Cytochromes b, c, d, and o were identified by spectroscopic analysis of respiratory membrane fragments from Vitreoscilla sp., strain C1. Carbon monoxide difference spectra of the reduced membranes had absorption maxima at 416, 534, and 571 nm (ascribed to cytochrome o) and 632 nm (cytochrome d). Derivative spectra of the pyridine hemochromogen spectra of the membranes identified the presence of b- and c-type cytochromes in Vitreoscilla. The cyanide binding curve of the membranes was biphasic with dissociation constants of 2.14 mM and 10.7 which were assigned to cytochrome o and cytochrome d, respectively. Membranes bound carbon monoxide with dissociation constant 3.9 microM, which was assigned to cytochrome o. Cytochrome c556 and a NADH-p-iodonitrotetrazolium violet reductase component were partially purified from Vitreoscilla membranes.
通过对维氏固氮菌C1菌株呼吸膜片段的光谱分析，鉴定出了细胞色素b、c、d和o。还原膜的一氧化碳差光谱在416、534和571nm（归属于细胞色素o）以及632nm（细胞色素d）处有吸收最大值。膜的吡啶血色原光谱的导数光谱确定了维氏固氮菌中存在b型和c型细胞色素。膜的氰化物结合曲线是双相的，解离常数分别为2.14mM和10.7，分别归属于细胞色素o和细胞色素d。膜与一氧化碳结合的解离常数为3.9μM，归属于细胞色素o。细胞色素c556和一种NADH - 对碘硝基四氮唑紫还原酶组分从维氏固氮菌膜中得到了部分纯化。
