Hagenbuch B, Stange G, Murer H
Department of Physiology, University of Zurich, Switzerland.
Biochem J. 1987 Sep 1;246(2):543-5. doi: 10.1042/bj2460543.
Basolateral membrane vesicles were isolated from rat kidney cortex and small intestinal enterocytes. Both membrane preparations show ATP-dependent calcium uptake and cytochalasin B-sensitive D-glucose transport. In renal membranes, sodium influx is stimulated by bicarbonate; bicarbonate-dependent sodium flux is membrane-potential-dependent and inhibited by 4,4'-di-isothiocyanato-2, 2'-stilbenedisulphanic acid ('DIDS'). Small intestinal basolateral membranes do not show bicarbonate-dependent sodium fluxes.
从大鼠肾皮质和小肠肠上皮细胞中分离出基底外侧膜囊泡。两种膜制剂均显示出ATP依赖性钙摄取和细胞松弛素B敏感的D-葡萄糖转运。在肾膜中，碳酸氢盐刺激钠内流；依赖碳酸氢盐的钠通量依赖于膜电位，并被4,4'-二异硫氰酸-2,2'-二苯乙烯二磺酸（“DIDS”）抑制。小肠基底外侧膜未显示出依赖碳酸氢盐的钠通量。
