Kucera I, Lampardová L, Dadák V
Department of Biochemistry, Faculty of Science, J.E. Purkynĕ University, Brno, Czechoslovakia.
Biochem J. 1987 Sep 15;246(3):779-82. doi: 10.1042/bj2460779.
By means of fluorimetric measurement and by direct determination of intracellular NAD+ and NADH contents, it was proved that the respiration rate of Paracoccus denitrificans cells utilizing glucose is limited by processes preceding NADH oxidation in the respiratory chain, so that the membrane NADH dehydrogenase is not saturated by its substrate. In the separated membrane fraction on saturation with exogenous NADH the main limiting factor is represented by NADH: ubiquinone oxidoreductase.
通过荧光测量以及直接测定细胞内NAD⁺和NADH的含量，已证明利用葡萄糖的反硝化副球菌细胞的呼吸速率受呼吸链中NADH氧化之前的过程限制，因此膜NADH脱氢酶未被其底物饱和。在外源NADH饱和的分离膜部分中，主要限制因素是NADH：泛醌氧化还原酶。
