细胞外ATP与仙台病毒诱导的通透化作用的比较研究。

A comparative study of permeabilization induced by extracellular ATP and by Sendai virus.

作者信息

Sternberg Z, Heppel L A

机构信息

Section of Biochemistry, Molecular and Cell Biology, Cornell University, Ithaca, NY 14853.

出版信息

Biochem Biophys Res Commun. 1987 Oct 29;148(2):560-6. doi: 10.1016/0006-291x(87)90913-2.

DOI:10.1016/0006-291x(87)90913-2

PMID:2825663

Abstract

Both Sendai virus and extracellular ATP induce membrane changes in 3T6 cells which allow passage of phosphorylated metabolites and normally impermeant aqueous solutes. The two processes share many features in common, including their kinetics and the effects of temperature, Ca2+, and various metabolic inhibitors. Furthermore, in each case permeabilization is preceded by net changes in intracellular cations. However, there are significant differences in that only ATP-dependent permeabilization is influenced by changes in the ionic strength of the medium, by inhibition of the Na+, K+, Cl- cotransporter and by preincubation of 3T6 cells with dithiothreitol.

摘要

仙台病毒和细胞外ATP都会诱导3T6细胞发生膜变化，这种变化允许磷酸化代谢产物和通常不能透过的水溶性溶质通过。这两个过程有许多共同特征，包括它们的动力学以及温度、Ca2+和各种代谢抑制剂的影响。此外，在每种情况下，通透化之前细胞内阳离子都会发生净变化。然而，它们之间也存在显著差异，即只有依赖ATP的通透化会受到培养基离子强度变化、Na+、K+、Cl-协同转运蛋白的抑制以及3T6细胞与二硫苏糖醇预孵育的影响。