细胞外ATP使转化细胞对大分子具有通透性。
External ATP permeabilizes transformed cells to macromolecules.
作者信息
Otero M J, Carrasco L
出版信息
Biochem Biophys Res Commun. 1986 Jan 29;134(2):453-60. doi: 10.1016/s0006-291x(86)80442-9.
PMID:3947333
Abstract
External ATP under certain ionic conditions render transformed cells permeable to the translation inhibitor hygromycin B. With this method the protein toxin alpha-sarcin selectively penetrates into 3T6 cells, as compared to 3T3 cells. This entry is enhanced by ATP synthesis blockers such as CCCP. Other proteins, such as horseradish peroxidase and luciferase, also pass selectively in 3T6 cells under permeabilization conditions.
摘要
在特定离子条件下，细胞外ATP可使转化细胞对翻译抑制剂潮霉素B具有通透性。用这种方法，与3T3细胞相比，蛋白质毒素α-肌动蛋白可选择性地渗透到3T6细胞中。像CCCP这样的ATP合成阻滞剂可增强这种进入。在通透条件下，其他蛋白质，如辣根过氧化物酶和荧光素酶，也能选择性地进入3T6细胞。
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