来自牛多形核白细胞的12-脂氧合酶，一种具有白三烯A4合酶活性的酶。

12-Lipoxygenase from bovine polymorphonuclear leukocytes, an enzyme with leukotriene A4-synthase activity.

作者信息

Walstra P, Verhagen J, Veldink G A, Vliegenthart J F

机构信息

Department of Bio-Organic Chemistry, State University of Utrecht, The Netherlands.

出版信息

Biochem Biophys Res Commun. 1987 Nov 30;149(1):258-65. doi: 10.1016/0006-291x(87)91633-0.

DOI:10.1016/0006-291x(87)91633-0

PMID:2825701

Abstract

Bovine polymorphonuclear leukocytes exhibit a 12-lipoxygenase activity upon sonication. In contrast to bovine platelet 12-lipoxygenase and other 12-lipoxygenases, this enzyme is unable to convert 5(S)-HETE (5(S)-hydroxy,6-trans-8,11,14-cis-eicosatetraenoic acid) or 5(S)-HPETE (5(S)-hydroperoxy,6-trans-8,11,14-cis-eicosatetraenoic acid) into 5(S),12(S)-dihydroxy-6,10-trans,8,14-cis-eicosatetraenoic acid. Surprisingly, the formation of leukotriene A4-derived products namely leukotriene B4 and the leukotriene B4-isomers 12-epi,6-trans- leukotriene B4 and 6-trans-leukotriene B4, was observed upon incubation of this enzyme with 5(S)-HPETE. Hence, the 12-lipoxygenase from bovine polymorphonuclear leukocytes possesses leukotriene A4-synthase activity.

摘要

牛多形核白细胞在超声处理后表现出12-脂氧合酶活性。与牛血小板12-脂氧合酶和其他12-脂氧合酶不同，这种酶无法将5(S)-HETE（5(S)-羟基，6-反式-8,11,14-顺式-二十碳四烯酸）或5(S)-HPETE（5(S)-氢过氧基，6-反式-8,11,14-顺式-二十碳四烯酸）转化为5(S),12(S)-二羟基-6,10-反式,8,14-顺式-二十碳四烯酸。令人惊讶的是，当该酶与5(S)-HPETE一起孵育时，观察到了白三烯A4衍生产物即白三烯B4以及白三烯B4异构体12-表位,6-反式-白三烯B4和6-反式-白三烯B4的形成。因此，来自牛多形核白细胞的12-脂氧合酶具有白三烯A4合酶活性。