Ueda N, Yamamoto S, Oates J A, Brash A R
Prostaglandins. 1986 Jul;32(1):43-8. doi: 10.1016/0090-6980(86)90141-3.
Arachidonate 5-lipoxygenase purified from porcine leukocytes transformed arachidonic acid to 5-hydroperoxy-6,8,11,14-eicosatetraenoic acid. By the leukotriene A synthase activity of the same enzyme the product was further metabolized to leukotriene A4 (actually detected as 6-trans-leukotriene B4, 12-epi-6-trans-leukotriene B4, and 5,6-dihydroxy-7,9,11,14-eicosatetraenoic acids). The enzyme was incubated with [10-DR-3H]- or [10-LS-3H]-labeled arachidonic acid, and 6-trans-LTB4 and its 12-epimer were analyzed. More than 90% of 10-DR-hydrogen was lost while about 100% of 10-LS-hydrogen was retained, indicating a stereospecific hydrogen elimination from C-10 during the formation of leukotriene A4.
从猪白细胞中纯化得到的花生四烯酸5-脂氧合酶将花生四烯酸转化为5-氢过氧-6,8,11,14-二十碳四烯酸。通过同一酶的白三烯A合酶活性，该产物进一步代谢为白三烯A4（实际检测为6-反式白三烯B4、12-表-6-反式白三烯B4和5,6-二羟基-7,9,11,14-二十碳四烯酸）。将该酶与[10-DR-3H]-或[10-LS-3H]-标记的花生四烯酸一起孵育，并分析6-反式白三烯B4及其12-差向异构体。超过90%的10-DR-氢丢失，而约100%的10-LS-氢保留下来，这表明在白三烯A4形成过程中，C-10位发生了立体特异性的氢消除。
