Rodrigues Nureyev F, Fonseca Guilherme C da, Kulcheski Franceli R, Margis Rogério
Departamento de Genética, PPGBM, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
Centro de Biotecnologia, PPGBCM, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
Genet Mol Biol. 2017;40(1 suppl 1):200-208. doi: 10.1590/1678-4685-GMB-2016-0055. Epub 2017 Mar 2.
Soybean, a crop known by its economic and nutritional importance, has been the subject of several studies that assess the impact and the effective plant responses to abiotic stresses. Salt stress is one of the main environmental stresses and negatively impacts crop growth and yield. In this work, the RNA editing process in the chloroplast of soybean plants was evaluated in response to a salt stress. Bioinformatics approach using sRNA and mRNA libraries were employed to detect specific sites showing differences in editing efficiency. RT-qPCR was used to measure editing efficiency at selected sites. We observed that transcripts of NDHA, NDHB, RPS14 and RPS16 genes presented differences in coverage and editing rates between control and salt-treated libraries. RT-qPCR assays demonstrated an increase in editing efficiency of selected genes. The salt stress enhanced the RNA editing process in transcripts, indicating responses to components of the electron transfer chain, photosystem and translation complexes. These increases can be a response to keep the homeostasis of chloroplast protein functions in response to salt stress.
大豆是一种因其经济和营养重要性而闻名的作物，它一直是多项评估非生物胁迫对其影响以及有效植物反应的研究对象。盐胁迫是主要的环境胁迫之一，会对作物生长和产量产生负面影响。在这项工作中，研究了大豆植株叶绿体中的RNA编辑过程对盐胁迫的响应。采用生物信息学方法，利用小RNA和信使RNA文库来检测编辑效率存在差异的特定位点。实时定量聚合酶链反应（RT-qPCR）用于测量选定位点的编辑效率。我们观察到，在对照文库和盐处理文库之间，NDHA、NDHB、RPS14和RPS16基因的转录本在覆盖率和编辑率上存在差异。RT-qPCR分析表明选定基因的编辑效率有所提高。盐胁迫增强了转录本中的RNA编辑过程，表明对电子传递链、光系统和翻译复合体的成分有反应。这些增加可能是对盐胁迫保持叶绿体蛋白质功能稳态的一种反应。
