Abdel-Naby Waleed, Cole Brigette, Liu Aihong, Liu Jingbo, Wan Pengxia, Guaiquil Victor H, Schreiner Ryan, Infanger David, Lawrence Brian D, Rosenblatt Mark I
Department of Biomedical Engineering, Cornell University, Ithaca, New York, United States 2Department of Ophthalmology, Weill Cornell Medical College, New York, New York, United States.
Department of Ophthalmology, Weill Cornell Medical College, New York, New York, United States.
Invest Ophthalmol Vis Sci. 2017 Mar 1;58(3):1425-1433. doi: 10.1167/iovs.16-19957.
The corneal surface is vulnerable to a myriad of traumatic insults including mechanical, chemical, and thermal injuries. The resulting trauma may render the naturally occurring regenerative properties of the cornea incapable of restoring a healthy epithelial surface, and may result in the loss of corneal transparency and vision. Healing of the corneal epithelium requires a complex cascade of biological processes that work to restore the tissue after injury. New therapeutic agents that act on the multiple steps of the corneal wound-healing process would offer a potential for improving patient outcomes. Here, a novel silk fibroin-derived protein (SDP) was studied for potential impacts on wound healing through studying an in vitro model.
Solubilized SDP, produced from the Bombyx mori silkworm cocoon, was added to human corneal limbal-epithelial (hCLE) cultures to evaluate the material's effects on epithelial cell migration, proliferation, and adhesion through the use of various scratch wound assays and flow chamber studies.
Results indicated that the addition of SDP to culture increased hCLE migration rate by over 50%, and produced an approximate 60% increase in cell proliferation. This resulted in a nearly 30% enhancement of in vitro scratch wound closure time. In addition, cultures treated with SDP experienced increased cell-matrix focal adhesion formation by over 95% when compared to controls.
The addition of SDP to culture media significantly enhanced hCLE cell sheet migration, proliferation, and attachment when compared to untreated controls, and indicates SDP's potential utility as an ophthalmic therapeutic agent.
角膜表面易受到多种创伤性损伤，包括机械性、化学性和热性损伤。由此产生的创伤可能使角膜天然的再生特性无法恢复健康的上皮表面，并可能导致角膜透明度和视力丧失。角膜上皮的愈合需要一系列复杂的生物过程，这些过程在损伤后发挥作用以恢复组织。作用于角膜伤口愈合过程多个步骤的新型治疗药物有望改善患者的治疗效果。在此，通过研究体外模型，对一种新型丝素蛋白衍生蛋白（SDP）在伤口愈合方面的潜在影响进行了研究。
将由家蚕茧制备的可溶性SDP添加到人类角膜缘上皮（hCLE）培养物中，通过各种划痕伤口试验和流动腔室研究来评估该材料对上皮细胞迁移、增殖和黏附的影响。
结果表明，向培养物中添加SDP可使hCLE迁移率提高超过50%，细胞增殖增加约60%。这导致体外划痕伤口闭合时间缩短近30%。此外，与对照组相比，用SDP处理的培养物中细胞-基质焦点黏附形成增加超过95%。
与未处理的对照组相比，向培养基中添加SDP可显著增强hCLE细胞片的迁移、增殖和附着，表明SDP作为眼科治疗药物的潜在效用。
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