Xu Jiejie, Peng Baogang, Zhang Caiyun, Xu Jiwei, Ma Yi, Lu Xinjun
a Department of Hepatic Surgery, the First Affiliated Hospital, Sun Yat-Sen University, No. 58 Zhongshan 2nd Road, Guangzhou 510080, P.R. China.
b Department of Hepatobiliary Surgery, Meizhou People's Hospital, No. 63 Huangtang Road, Meizhou 514000, P.R. China.
Biochem Cell Biol. 2017 Aug;95(4):455-458. doi: 10.1139/bcb-2016-0204. Epub 2017 Mar 3.
Mouse islets are widely used in diabetes research. Thus an adequate quality, quantity, and purity of islets are needed for high-quality investigations. We performed a combination of filtration and density gradient separation and optimized many steps in the islet isolation procedure, including perfusion, digestion, and purification. Our results show that an increased quality, quantity, and purity of isolated islets can be achieved using these modifications. Moreover, this method can guarantee maximal recovery and purity of the isolated islets and is easy to perform with practice.
小鼠胰岛广泛应用于糖尿病研究。因此，高质量的研究需要具备足够质量、数量和纯度的胰岛。我们进行了过滤和密度梯度分离相结合的操作，并优化了胰岛分离过程中的许多步骤，包括灌注、消化和纯化。我们的结果表明，通过这些改进可以提高分离出的胰岛的质量、数量和纯度。此外，这种方法可以保证分离出的胰岛的最大回收率和纯度，并且通过实践操作很容易实施。
