Shaik Haq Abdul, Mishra Archana, Kodrík Dalibor
Institute of Entomology, Biology Centre, CAS, Branišovská 31, 370 05 České Budějovice, Czech Republic.
Institute of Entomology, Biology Centre, CAS, Branišovská 31, 370 05 České Budějovice, Czech Republic; Faculty of Science, University of South Bohemia, Branišovská 31, 370 05 České Budějovice, Czech Republic.
Comp Biochem Physiol C Toxicol Pharmacol. 2017 Jun;196:11-18. doi: 10.1016/j.cbpc.2017.02.011. Epub 2017 Feb 28.
The effect of Habrobracon hebetor venom and the role of the adipokinetic hormone (AKH) in poisoned adult females of the firebug Pyrrhocoris apterus were studied 24 and 48h after treatments. Venom application elicited total neuromuscular paralysis in firebugs, but the co-application of venom and Pyrap-AKH significantly reduced paralysis (up to 3.2 times) compared to the application of venom only. Although the mechanisms of their action are unknown, both agents might affect neuromuscular junctions. Venom application significantly increased the expression of both P. apterus Akh genes (Pyrap-Akh 5.4 times and Peram-Cah-II 3.6 times), as well as the level of AKHs in the central nervous system (2.5 times) and haemolymph (3.0 times). In the haemolymph, increased AKH levels might have led to the mobilization of stored lipids, which increased 1.9 times, while the level of free carbohydrates remained unchanged. Total metabolism, monitored by carbon dioxide production, significantly declined in paralysed P. apterus individuals (1.4 times and 1.9 times, 24 and 48h after the treatment, respectively), probably because of a malfunction of the muscular system. The results suggest an active role of AKH in the defence mechanism against the stress elicited by neuromuscular paralysis, and the possible involvement of this hormone in neuronal/neuromuscular signalling.
在处理后的24小时和48小时，研究了哈氏肿腿蜂毒液对红蝽成年雌性个体的影响以及脂肪动激素（AKH）在中毒个体中的作用。涂抹毒液会引发红蝽完全的神经肌肉麻痹，但与仅涂抹毒液相比，毒液与红蝽AKH（Pyrap-AKH）共同涂抹可显著减轻麻痹（最多达3.2倍）。尽管它们的作用机制尚不清楚，但两种药剂可能都会影响神经肌肉接头。涂抹毒液显著增加了红蝽Akh基因（Pyrap-Akh增加5.4倍，Peram-Cah-II增加3.6倍）的表达，以及中枢神经系统中AKH的水平（增加2.5倍）和血淋巴中AKH的水平（增加3.0倍）。在血淋巴中，AKH水平的升高可能导致储存脂质的动员，脂质增加了1.9倍，而游离碳水化合物的水平保持不变。通过二氧化碳产生监测的总代谢在麻痹的红蝽个体中显著下降（处理后24小时和48小时分别下降1.4倍和1.9倍），这可能是由于肌肉系统功能失调所致。结果表明，AKH在抵抗神经肌肉麻痹引发的应激的防御机制中发挥积极作用，并且这种激素可能参与神经元/神经肌肉信号传导。
