黄芩素对人多形核白细胞白三烯生物合成及脱颗粒的影响。

Effects of baicalein on leukotriene biosynthesis and degranulation in human polymorphonuclear leukocytes.

作者信息

Kimura Y, Okuda H, Arichi S

机构信息

Second Department of Medical Biochemistry, School of Medicine, Ehime University, Japan.

出版信息

Biochim Biophys Acta. 1987 Dec 14;922(3):278-86.

PMID:2825795

Abstract

Studies were made on the effects of baicalein (5,6,7-trihydroxyflavone) on leukotrienes B4 and C4 biosyntheses and degranulation induced by calcium ionophore A23187 (A23187) in human polymorphonuclear leukocytes. Baicalein inhibited A23187-induced biosynthesis of leukotrienes B4 and C4 in human polymorphonuclear leukocytes. The concentration of baicalein required for 50% inhibition (IC50) of leukotrienes B4 and C4 formations was 1.46.10(-6) and 6.00.10(-7) M, respectively, using 1.0 microgram/ml of A23187. In addition, baicalein dose-dependently inhibited beta-glucuronidase and lysozyme releases induced by A23187, leukotriene B4 plus cytochalasin B and platelet-activating factor plus cytochalasin B. Furthermore, baicalein was found to inhibit dose-dependently Ca2+ uptake into the cells and Ca2+ mobilization from the intracellular stores.

摘要

研究了黄芩苷（5,6,7 - 三羟基黄酮）对人多形核白细胞中白三烯B4和C4生物合成以及钙离子载体A23187（A23187）诱导的脱颗粒作用。黄芩苷抑制人多形核白细胞中A23187诱导的白三烯B4和C4生物合成。当使用1.0微克/毫升的A23187时，对白三烯B4和C4形成产生50%抑制（IC50）所需的黄芩苷浓度分别为1.46×10⁻⁶和6.00×10⁻⁷ M。此外，黄芩苷剂量依赖性地抑制A23187、白三烯B4加细胞松弛素B以及血小板活化因子加细胞松弛素B诱导的β - 葡萄糖醛酸酶和溶菌酶释放。此外，发现黄芩苷剂量依赖性地抑制细胞对Ca²⁺的摄取以及细胞内储存库中Ca²⁺的动员。